VIRAL INDUCTION OF LOW-FREQUENCY INTERFERON-ALPHA PRODUCING CELLS
VIRAL INDUCTION OF LOW-FREQUENCY INTERFERON-ALPHA PRODUCING CELLS
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DOI：
10.1006/viro.1994.1504
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发表时间：
1994-10-01
期刊：
影响因子：
3.7
通讯作者：
FITZGERALDBOCARSLY, P
中科院分区：
文献类型：
--
作者：
FELDMAN, SB;FERRARO, M;FITZGERALDBOCARSLY, P
负责响应病毒刺激而产生 IFN-α 的人外周血单核细胞最常被描述为单核细胞（以对仙台病毒的反应为代表）或轻密度 HLA-DR(+) 群体，其对成熟 T 细胞、B 细胞、单核细胞或自然杀伤细胞的大多数细胞表面标志物特征呈阴性（以对单纯疱疹病毒 (HSV) 的反应为代表）。产生 IFN-α 的细胞 (IPC) 对仙台病毒作出反应的频率通常比对 HSV 作出反应的细胞高 10 倍或更多。在当前的研究中，我们使用 ELISpot 检测来确定 IPC 对 DNA 和 RNA 病毒的响应频率，包括 HSV、仙台病毒、水泡性口炎病毒、巨细胞病毒、腺病毒、SV40、流感、麻疹、腮腺炎、新城疫病毒 (NDV) 和人类免疫缺陷病毒 (HIV)。有包膜病毒而非无包膜病毒（腺病毒和 SV40）引发 IFN-α 反应。其他每种病毒的 IPC 频率与低频 HSV 反应人群比与高频仙台病毒反应更相似。其中包括与仙台病毒属于同一科的几种病毒，即副粘病毒麻疹、腮腺炎和新城疫病毒。 IPC 还测试了对溶酶体药物氯喹的敏感性，该药物会减少针对 HSV 而非仙台病毒产生的 IFN-α。除仙台病毒外，氯喹治疗消除了大部分产生的 IFN-α 和针对每种病毒的 IPC。我们得出的结论是，低频率、非单核细胞 NIPC 占响应不同病毒而产生的 IFN-α 的大部分。 (C) 1994 年学术出版社
The human peripheral blood mononuclear cells responsible for IFN-alpha production in response to viral stimuli have been most often described as either monocytes (as typified by the response to Sendai virus) or as a light density, HLA-DR(+) population which is negative for most cell surface markers characteristic of mature T cells, B cells, monocytes, or natural killer cells (as typified by the response to Herpes simplex virus (HSV)). The frequency of IFN-alpha-producing cells (IPC) responding to Sendai virus is typically 10-fold or more higher than those responding to HSV. In the current study, we have used ELISpot assays to determine the frequency of IPC responding to DNA and RNA viruses including HSV, Sendai, vesicular stomatitis virus, cytomegalovirus, adenovirus, SV40, influenza, measles, mumps, Newcastle disease virus (NDV) and human immunodeficiency virus (HIV). The enveloped viruses but not the nonenveloped viruses (adenovirus and SV40) elicited an IFN-alpha response. The frequency of IPC for each of the other viruses was more similar to the low frequency HSV-responding population than to the higher frequency Sendai virus response. These included several viruses in the same family as Sendai virus, namely the paramyxo viruses measles, mumps, and NDV. IPC were also tested for sensitivity to the lysosomotropic drug chloroquine, which diminishes IFN-alpha produced in response to HSV but not Sendai virus. With the exception of Sendai virus, chloroquine treatment abrogated the majority of IFN-alpha produced and IPC against each of the viruses. We conclude that low frequency, nonmonocytic NIPC account for the majority of IFN-alpha production in response to different viruses. (C) 1994 Academic Press, Inc.