RNA polymerase II structure: from core to functional complexes
RNA polymerase II structure: from core to functional complexes
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DOI：
10.1016/j.gde.2004.01.003
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发表时间：
2004-04-01
影响因子：
4
通讯作者：
Cramer, P
中科院分区：
文献类型：
--
作者：
Cramer, P
RNA聚合酶II（Pol II）复合物的新结构研究标志着真核生物mRNA转录周期的详细机制分析的开始。完整的Pol II的晶体学模型，加上新的生化和电子显微镜数据，提供了对转录起始的见解。第一个X射线分析的Pol II复合物与转录因子，延伸因子THS，支持的想法，聚合酶有一个“可调”的活性位点之间切换mRNA的合成和切割。新的研究还表明，转录因子的结构域可以进入聚合酶开口，以调节转录过程中的功能。
New structural studies of RNA polymerase II (Pol II) complexes mark the beginning of a detailed mechanistic analysis of the eukaryotic mRNA transcription cycle. Crystallographic models of the complete Pol II, together with new biochemical and electron microscopic data, give insights into transcription initiation. The first X-ray analysis of a Pol II complex with a transcription factor, the elongation factor THS, supports the idea that the polymerase has a 'tunable' active site that switches between mRNA synthesis and cleavage. The new studies also show that domains of transcription factors can enter polymerase openings, to modulate function during transcription.