(E)-Nerolidol is a volatile signal that induces defenses against insects and pathogens in tea plants
(E)-Nerolidol is a volatile signal that induces defenses against insects and pathogens in tea plants
复制标题
DOI：
10.1038/s41438-020-0275-7
复制
发表时间：
2020-04-01
影响因子：
8.7
通讯作者：
Xin, Zhaojun
中科院分区：
文献类型：
--
作者：
Chen, Shenglong;Zhang, Liping;Xin, Zhaojun
植物释放大量挥发性有机化合物（VOCs）来应对攻击者。几种挥发性有机化合物可以作为挥发性信号引起未受损组织和邻近植物的防御反应，但关于挥发性有机化合物的生态功能的许多问题尚未得到解答。茶树受到两种有害入侵者的侵袭，即刺穿性草食动物松田肉苁茸和病原菌松果炭疽菌。为了确定茶叶中挥发性有机化合物（VOC）的信号，我们确定了copr3是一个标记基因，并建立了一种基于拟南芥中1.51 kb的copr3启动子与β -葡萄糖醛酸酶（GUS）报告基因（OPR3p::GUS）融合的快速筛选方法。使用这种筛选系统，萜类挥发物(E)-神经醇被确定为引发植物防御的有效信号。(E)-神经树醇引发的早期反应包括激活丝裂原激活的蛋白激酶和WRKY， H2O2爆发，诱导茉莉酸和脱落酸信号传导。诱导植株积累了高水平的具有广谱抗草食或抗病原菌特性的防御相关化学物质，并最终引发了茶叶对肉麻病菌和果炭疽病菌的抗性。我们认为这些发现可以为茶树病虫害的防治提供一种环境友好型管理策略。
Plants release large amounts of volatile organic compounds (VOCs) in response to attackers. Several VOCs can serve as volatile signals to elicit defense responses in undamaged tissues and neighboring plants, but many questions about the ecological functions of VOCs remain unanswered. Tea plants are impacted by two harmful invaders, the piercing herbivore Empoasca (Matsumurasca) onukii Matsuda and the pathogen Colletotrichum fructicola. To determine the VOC signals in tea, we confirmed CsOPR3 as a marker gene and set up a rapid screening method based on a 1.51 kb CsOPR3 promoter fused with a beta-glucuronidase (GUS) reporter construct (OPR3p::GUS) in Arabidopsis. Using this screening system, a terpenoid volatile (E)-nerolidol was identified as a potent signal that elicits plant defenses. The early responses triggered by (E)-nerolidol included the activation of a mitogen-activated protein kinase and WRKY, an H2O2 burst, and the induction of jasmonic acid and abscisic acid signaling. The induced plants accumulated high levels of defense-related chemicals, which possessed broad-spectrum anti-herbivore or anti-pathogen properties, and ultimately triggered resistance against Empoasca onukii and Colletotrichum fructicola in tea. We propose that these findings can supply an environmentally friendly management strategy for controlling an insect pest and a disease of tea plants.