Effects of heat stress and starvation on clonal odontoblast-like cells.
Effects of heat stress and starvation on clonal odontoblast-like cells.
复制标题
DOI：
10.1016/j.joen.2011.03.037
复制
发表时间：
2011-07
影响因子：
4.2
通讯作者：
T. Morotomi;C. Kitamura;T. Toyono;T. Okinaga;A. Washio;Noriko Saito;T. Nishihara;M. Terashita;H. Anan
中科院分区：
文献类型：
--
作者：
T. Morotomi;C. Kitamura;T. Toyono;T. Okinaga;A. Washio;Noriko Saito;T. Nishihara;M. Terashita;H. Anan
在修复过程中的热应激，特别是在严重饥饿的条件下，会引发牙髓损伤。在本研究中，我们研究了热应激对成牙本质细胞活性和炎症反应的影响，在成牙本质细胞样细胞系（KN-3）血清饥饿conditions.MethodsViability，核结构，和KN-3细胞的炎症反应进行了检查，在培养基中含有10%或1%的血清暴露后，在43°C的热应激45分钟。细胞外基质的基因表达，碱性磷酸酶的活性，并检测细胞外钙沉积暴露于热stress.ResultsReduced活力和凋亡的细胞在KN-3细胞在热应激后的初始阶段瞬时诱导，此后，细胞恢复其活力。热应激的细胞毒作用在血清饥饿条件下增强。热应激也强烈上调KN-3细胞中热休克蛋白25的表达以及肿瘤坏死因子-α、白细胞介素-6和环氧合酶-2的瞬时表达。相比之下，1型胶原，runt相关转录因子2，和牙本质sialophoprotein的表达没有受到抑制，热应激，虽然饥饿抑制ALP活性和延迟进展calcium.ConclusionsOdontoblast-like细胞表现出耐热性与短暂的炎症反应和没有损失的钙化活性，和他们的耐热性和钙化活性的影响，营养状况。
IntroductionHeat stress during restorative procedures, particularly under severe starvation conditions, can trigger damage to dental pulp. In the present study, we examined effects of heat stress on odontoblastic activity and inflammatory responses in an odontoblast-like cell line (KN-3) under serum-starved conditions.MethodsViability, nuclear structures, and inflammatory responses of KN-3 cells were examined in culture medium containing 10% or 1% serum after exposure to heat stress at 43°C for 45 minutes. Gene expression of extracellular matrices, alkaline phosphatase activity, and detection of extracellular calcium deposition in cells exposed to heat stress were also examined.ResultsReduced viability and apoptosis were transiently induced in KN-3 cells during the initial phases after heat stress; thereafter, cells recovered their viability. The cytotoxic effects of heat stress were enhanced under serum-starved conditions. Heat stress also strongly up-regulated expression of heat shock protein 25 as well as transient expression of tumor necrosis factor-alpha, interleukin-6, and cyclooxygenase-2 in KN-3 cells. In contrast, expression of type-1 collagen, runt-related transcription factor 2, and dentin sialophosphoprotein were not inhibited by heat stress although starvation suppressed ALP activity and delayed progression of calcification.ConclusionsOdontoblast-like cells showed thermoresistance with transient inflammatory responses and without loss of calcification activity, and their thermoresistance and calcification activity were influenced by nutritional status.