Cytochemical observations on the chromatoid body of the male germ cells.
Cytochemical observations on the chromatoid body of the male germ cells.
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DOI：
10.1095/biolreprod2.1.114
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发表时间：
1970-01-01
期刊：
影响因子：
--
通讯作者：
Eddy, E. M.
中科院分区：
文献类型：
--
作者：
Eddy, E. M.
早期关于哺乳动物类染色体中存在大量核糖核酸的报道，是基于利用核糖核酸酶消化和切片上的优先染色获得的证据，然后用光学显微镜进行检查。在本研究中，几种被认为是在电子显微镜水平上显示核酸的细胞化学技术提供了证据，表明核糖核酸不存在于类染色体中。此外，用链霉蛋白酶或胃酶消化薄片，以确定颈部或鞭毛的后期形成结构是否具有与类染色体类似的对这些酶的敏感性。拟色素体抵抗酶消化，而鞭毛的连接件和外部致密纤维的节段柱被提取。特别是，这使得文献中关于类色质小体可能产生连接件的建议变得有点不太可能。
Earlier reports indicating the presence of significant amounts of ribonucleic acid in the chromatoid body of mammals were based on evidence obtained by using ribonuclease digestion and preferential stains on sections subsequently examined with the light microscope. In the present study several cytochemical techniques believed to be specific for the demonstration of nucleic acids at the electron microscope level provided evidence which indicates that ribonucleic acid is not present in the chromatoid body. Additionally, thin sections were digested with pronase or pepsin to determine if later forming structures of the neck or flagellum possess similar susceptibility to these enzymes as the chromatoid body. The chromatoid body resisted enzymatic digestion while the segmented columns of the connecting piece and outer dense fibers of the flagellum were extracted. In particular, this makes somewhat unlikely the suggestion in the literature that the chromatoid body may give rise to the connecting piece.