dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms:: experimental applications in Arabidopsis thaliana genetics
dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms:: experimental applications in Arabidopsis thaliana genetics
复制标题
DOI：
10.1046/j.1365-313x.1998.00124.x
复制
发表时间：
1998-05-01
期刊：
影响因子：
7.2
通讯作者：
Pepper, AE
中科院分区：
文献类型：
--
作者：
Neff, MM;Neff, JD;Pepper, AE
基于聚合酶链式反应的单核苷酸多态检测是植物遗传学家的有力工具。裂解扩增多态序列分析是目前应用最广泛的单核苷酸多态检测方法。然而，这项技术仅限于产生或破坏限制酶识别位点的突变。本文介绍了这项技术的一种改进，其中利用聚合酶链式反应中的不匹配来创建基于目标突变的多态。这项技术对于跟踪分离群体中的已知突变和使用基于远位置克隆新基因的分离DNA的遗传图谱是有用的。此外，还开发了一种计算机程序，以便于设计这些聚合酶链式反应引物。
PCR-based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis is the most widely used approach for the detection of single nucleotide polymorphisms. However, this technique is limited to mutations which create or disrupt a restriction enzyme recognition site. This paper presents a modification of this technique where mismatches in a PCR primer are used to create a polymorphism based on the target mutation. This technique is useful for following known mutations in segregating populations and genetic mapping of isolated DNAs used far positional based cloning of new genes. In addition, a computer program has been developed that facilitates the design of these PCR primers.