Upregulation of plasma CCL8 in mouse model of graft-vs-host disease
Upregulation of plasma CCL8 in mouse model of graft-vs-host disease
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DOI：
10.1016/j.exphem.2008.12.006
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发表时间：
2009-04-01
影响因子：
2.6
通讯作者：
Kokai, Yasuo
中科院分区：
文献类型：
--
作者：
Ota, Akinobu;Yamamoto, Masaki;Kokai, Yasuo
客观的。使用蛋白质组学方法，我们最近发现血浆 CCL8 作为诊断小鼠和人类移植物抗宿主病 (GVHD) 的潜在生物标志物。由于质谱分析只是半定量，因此需要一种测量小鼠血浆 CCL8 水平的定量方法。材料和方法。我们建立了一种酶联免疫吸附测定法，用于定量测量小鼠血浆中的 CCL8 浓度。结果。我们新建立的酶联免疫吸附测定显示，同种异体骨髓移植（BMT）后第7天和第14天血浆CCL8浓度（平均标准误差；n = 12）分别为1287 +/- 55.7 ng/mL和1604 +/- 110.8 ng/mL，而血浆浓度为316.6 +/- 16.3 同基因 BMT 后第 7 天为 ng/mL。蛋白质印迹分析还显示，同种异体 BMT 组和同系 BMT 组之间血浆 CCL8 水平存在差异，临床 GVHD 评分也存在差异。脂多糖和聚 (I:C) 均未升高血浆 CCL8 浓度，尽管两种治疗后检测到白细胞介素 6 显着增加。结论。血浆 CCL8 浓度升高可能是小鼠模型中 GVHD 的一个有前途的血浆标志物。 (C) 2009 ISEH - 血液学和干细胞学会。由爱思唯尔公司出版
Objective. Using a proteomic approach, we recently identified plasma CCL8 as a potential biomarker for diagnosis of graft-vs-host-disease (GVHD) in mice as well as humans. Because mass spectrometric analysis is only semi-quantitative, a quantitative method of measuring plasma CCL8 levels in mice is needed.Materials and Methods. We established an enzyme-linked immunosorbent assay for the quantitative measurement of CCL8 concentrations in mouse plasma.Results. Our newly established enzyme-linked immunosorbent assay revealed that the plasma CCL8 concentrations (mean standard error; n = 12) were 1287 +/- 55.7 ng/mL and 1604 +/- 110.8 ng/mL on days 7 and 14 after allogeneic bone marrow transplantation (BMT), respectively, while the plasma concentrations was 316.6 +/- 16.3 ng/mL on day 7 after syngeneic BMT. A Western blotting analysis also showed a difference in the plasma CCL8 levels between the allogeneic and syngeneic BMT groups, as did clinical GVHD scores. Neither lipopolysaccharide nor poly(I:C) elevated the plasma CCL8 concentrations, although a dramatic increase in interleukin-6 was detected after both treatments.Conclusion. An elevated plasma CCL8 concentration may be a promising plasma marker for GVHD in mouse models. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.