Ablation of insulin-producing neurons in flies: Growth and diabetic phenotypes
Ablation of insulin-producing neurons in flies: Growth and diabetic phenotypes
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DOI：
10.1126/science.1070058
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发表时间：
2002-05-10
期刊：
影响因子：
56.9
通讯作者：
Nusse, R
中科院分区：
文献类型：
--
作者：
Rulifson, EJ;Kim, SK;Nusse, R
在果蝇（Drosophila）中，四种胰岛素基因在大脑中的小簇细胞（胰岛素产生细胞（IPC））中共同表达。在这里，我们表明，消融这些IPC导致发育迟缓，生长迟缓，并在幼虫血淋巴中的碳水化合物水平升高。所有的缺陷被逆转的果蝇胰岛素转基因的异位表达。根据这些功能数据和观察，IPC释放胰岛素到循环系统中，我们得出结论，脑IPC是主要的系统供应的胰岛素在幼虫的生长。我们认为，IPC和胰岛β细胞在功能上是相似的，可能是从一个共同的祖先产生胰岛素的神经元进化而来的。有趣的是，缺乏IPC的果蝇的表型包括糖尿病的某些特征。
In the fruit fly Drosophila, four insulin genes are coexpressed in small clusters of cells [insulin-producing cells (IPCs)] in the brain. Here, we show that ablation of these IPCs causes developmental delay, growth retardation, and elevated carbohydrate levels in larval hemolymph. All of the defects were reversed by ectopic expression of a Drosophila insulin transgene. On the basis of these functional data and the observation that IPCs release insulin into the circulatory system, we conclude that brain IPCs are the main systemic supply of insulin during larval growth. We propose that IPCs and pancreatic islet beta cells are functionally analogous and may have evolved from a common ancestral insulin-producing neuron. Interestingly, the phenotype of flies lacking IPCs includes certain features of diabetes mellitus.