SEQUENTIAL FUNCTION OF GENE PRODUCTS RELATIVE TO DNA-SYNTHESIS IN YEAST-CELL CYCLE
SEQUENTIAL FUNCTION OF GENE PRODUCTS RELATIVE TO DNA-SYNTHESIS IN YEAST-CELL CYCLE
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DOI：
10.1016/0022-2836(76)90183-2
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
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作者：
HARTWELL, LH
破译的相对依赖性的步骤在发育途径的实验原理，以确定之间的关系，在酿酒酵母细胞周期中的羟基脲敏感的步骤和各种温度敏感的步骤。由于羟基脲抑制酵母中的DNA复制，因此数据鉴定了DNA复制依赖于其功能的基因产物（cdc 4，6，7，2，8，21）和其功能或合成需要DNA复制的基因产物（cdc 2，8，21，9，13，16，23，5，15）。其他基因产物（cdc 3，11，24）的功能独立于DNA复制。这些结果表明，细胞周期的事件发生在一个规定的顺序，因为许多基因产物，介导这些事件被限制在一个规定的功能序列。2个基因（cdc 2和6）中的突变导致细胞在限制性温度下孵育后对羟基脲保持敏感，尽管这两种突变体在限制性温度下将放射性前体掺入DNA中。这表明，cdc 6指定了一个功能，这是必要的DNA复制的正确启动，和cdc 2的功能，这是必要的正确的DNA延伸，并在这些功能的情况下，所做的DNA是错误的或不完整的。
An experimental rationale for deciphering the relative dependence of steps in a developmental pathway was employed to determine the relationship between the hydroxyurea-sensitive step and various temperature-sensitive steps in the cell cycle of Saccharomyces cerevisiae. Since hydroxyurea inhibits DNA replication in yeast the data identify gene products upon whose function DNA replication is dependent (cdc 4, 6, 7, 2, 8, 21) and gene products whose function or synthesis requires DNA replication (cdc 2, 8, 21, 9, 13, 16, 23, 5, 15). Other gene products (cdc 3, 11, 24) function independent of DNA replication. These results suggest that the events of the cell cycle occur in a prescribed order because many of the gene products that mediate these events are restricted to a prescribed sequence of function. Mutations in 2 genes (cdc 2 and 6) result in cells that remain sensitive to hydroxyurea after an incubation at the restrictive temperature, despite the fact that both mutants incorporate radioactive precursors into DNA at the restrictive temperature. It is suggested that cdc 6 specifies a function that is necessary for the proper initiation of DNA replication, and cdc 2 a function that is necessary for correct DNA elongation, and that in the absence of either of these functions the DNA that is made is either faulty or incomplete.