THE ROLE OF CD44, CD45, CD45RO, CD46 AND CD55 AS POTENTIAL ANTIADHESION MOLECULES INVOLVED IN THE BINDING OF HUMAN TONSILLAR T-CELLS TO PHORBOL 12-MYRISTATE 13-ACETATE-DIFFERENTIATED U-937 CELLS
THE ROLE OF CD44, CD45, CD45RO, CD46 AND CD55 AS POTENTIAL ANTIADHESION MOLECULES INVOLVED IN THE BINDING OF HUMAN TONSILLAR T-CELLS TO PHORBOL 12-MYRISTATE 13-ACETATE-DIFFERENTIATED U-937 CELLS
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DOI：
10.1002/eji.1830200220
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发表时间：
1990-02-01
影响因子：
5.4
通讯作者：
KATZ, DR
中科院分区：
文献类型：
--
作者：
KING, PD;BATCHELOR, AH;KATZ, DR
在这项研究中，我们已经表明，人扁桃体T细胞粘附佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）分化的U-937细胞。为了检查所涉及的分子机制，在定量结合测定中评估了一组单克隆抗体对这种粘附的影响。针对LFA-1和ICAM-1的抗体抑制结合，直接暗示这些分子参与T细胞-PMA诱导的U-937粘附。此外，粘附是镁而不是钙依赖性的。在测试的其余抗体中，抗CD 2、LFA-3、Mac-1、p150、95、CD 43、CD 45 RA或CD 56的抗体均不影响结合。然而，针对CD 44、CD 45、CD 45 RO、CD 46和CD 55的抗体增强结合，表明这些分子在U-937-T细胞相互作用期间具有抗粘附作用。
In this study, we have shown that human tonsillar T cells adhere to phorbol 12-myristate 13-acetate(PMA)-differentiated U-937 cells. To examine the molecular mechanisms involved, the effect of a panel of monoclonal antibodies upon this adhesion was assessed in a quantitative binding assay. Antibodies against LFA-1 and ICAM-1 inhibited binding, directly implicated these molecules in T cell-PMA-induced U-937 adhesion. Furthermore, the adhesion was magnesium but not calcium dependent. Of the remaining antibodies that were tested, none of those against CD2, LFA-3, Mac-1, p150,95, CD43, CD45RA or CD56 affected binding. However, antibodies against CD44, CD45, CD45RO, CD46 and CD55 enhanced binding suggesting an anti-adhesive role for these molecules during U-937-T cell interaction.