The effects of NO2 on ionic surface charge on type I pneumocytes of hamster lungs.
The effects of NO2 on ionic surface charge on type I pneumocytes of hamster lungs.
复制标题
NO2 对仓鼠肺 I 型肺细胞离子表面电荷的影响。
DOI：
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发表时间：
1985
影响因子：
6
通讯作者：
R. Gordon
中科院分区：
文献类型：
--
作者：
R. Gordon
胞饮小泡是I型肺细胞胞质中常见的细胞器。未涂层囊泡的数量增加，在急性暴露于NO2。这些未涂层囊泡内吞辣根过氧化物酶。在这项研究中，目的是确定质膜表面特性诱导的NO2暴露对仓鼠肺的I型肺细胞。阳离子铁蛋白和钌红被用作离子表面探针。在暴露于NO2和阳离子铁蛋白后，通过戊二醛血管灌注在体内固定的肺组织薄切片上进行透射电子显微镜检查。暴露和固定的组织也用阳离子铁蛋白或钌红处理。戊二醛固定的I型肺细胞的透射电子显微镜检查证明了包被和未包被的囊泡。阳离子铁蛋白，它通常结合保守控制I型肺细胞，有一个高亲和力的I型肺细胞的质膜NO2暴露后。许多结合位点与囊泡的基础上存在的结合阳离子铁蛋白在开发和形成的囊泡。在与铁蛋白孵育30分钟的仓鼠肺中邻近基底层和基底层上的囊泡中也观察到阳离子铁蛋白壁。这表明铁蛋白通过未涂覆的囊泡转运穿过上皮。钌红，另一种阳离子分子，被发现在相同的网站作为阳离子铁蛋白。数据表明，NO2诱导的离子表面电荷的变化，这可能有助于未涂层的胞饮囊泡的形成。
Pinocytotic vesicles have been found to be common organelles throughout the cytoplasm of Type I pneumocytes. The number of uncoated vesicles were increased in response to acute exposure to NO2. These uncoated vesicles endocytosed horseradish peroxidase. In this study, the aim was to determine plasma membrane surface properties which were induced by NO2 exposure on the Type I pneumocytes of hamster lungs. Cationic ferritin and ruthenium red were used as ionic surface probes. Transmission electron microscopy was performed on thin sections of lung tissue fixed in vivo by vascular perfusion with glutaraldehyde after exposure to NO2 and cationic ferritin. Tissues exposed and fixed were also treated with cationic ferritin or ruthenium red. Transmission electron microscopic examination of glutaraldehyde-fixed Type I pneumocytes demonstrated both coated and uncoated vesicles. Cationic ferritin, which normally binds sparingly to control Type I pneumocytes, had a high affinity for the plasma membranes of Type I pneumocytes after NO2 exposure. Many of the binding sites were associated with vesicles on the basis of presence of bound cationic ferritin in developing and formed vesicles. Cationic ferritin wals also observed in vesicles adjacent to and on the basal lamina in the lungs from hamsters incubated with ferritin for 30 minutes. This indicated that the ferritin is transported across the epithelium by the uncoated vesicles. Ruthenium red, another cationic molecule, was found in the same sites as the cationic ferritin. The data showed that NO2 induced a change in the ionic surface charge, which may have contributed to the formation of uncoated pinocytotic vesicles.