Polarization of the C-elegans embryo by RhoGAP-mediated exclusion of PAR-6 from cell contacts
Polarization of the C-elegans embryo by RhoGAP-mediated exclusion of PAR-6 from cell contacts
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DOI：
10.1126/science.1156063
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发表时间：
2008-06-27
期刊：
影响因子：
56.9
通讯作者：
Nance, Jeremy
中科院分区：
文献类型：
--
作者：
Anderson, Dorian C.;Gill, Jason S.;Nance, Jeremy
一些后生动物的早期胚胎呈放射状排列，以促进关键的模式化事件，如原肠胚形成和不对称细胞分裂;然而，对放射极性是如何建立的知之甚少。秀丽隐杆线虫的早期胚胎在细胞接触时呈放射状排列，极性蛋白PAR- 6限制在无接触的细胞表面，在那里PAR- 6调节原肠胚的运动。我们已经鉴定了一种Rho鸟苷三磷酸酶激活蛋白（RhoGAP）PAC- 1，它介导C. elegans径向极性和原肠胚形成的细胞表面的PAR- 6排除。我们表明，PAC- 1被招募到细胞接触，我们认为，PAC- 1控制径向极性的限制活性CDC- 42接触自由的表面，CDC- 42结合和招聘PAR- 6。因此，PAC- 1提供了细胞接触和PAR蛋白之间的动态分子联系，使胚胎径向极化。
Early embryos of some metazoans polarize radially to facilitate critical patterning events such as gastrulation and asymmetric cell division; however, little is known about how radial polarity is established. Early embryos of Caenorhabditis elegans polarize radially when cell contacts restrict the polarity protein PAR- 6 to contact- free cell surfaces, where PAR- 6 regulates gastrulation movements. We have identified a Rho guanosine triphosphatase activating protein ( RhoGAP), PAC- 1, which mediates C. elegans radial polarity and gastrulation by excluding PAR- 6 from contacted cell surfaces. We show that PAC- 1 is recruited to cell contacts, and we suggest that PAC- 1 controls radial polarity by restricting active CDC- 42 to contact- free surfaces, where CDC- 42 binds and recruits PAR- 6. Thus, PAC- 1 provides a dynamic molecular link between cell contacts and PAR proteins that polarizes embryos radially.