TEMPERATURE EFFECT ON THE CILIARY BEAT FREQUENCY OF HUMAN NASAL AND TRACHEAL CILIATED CELLS
TEMPERATURE EFFECT ON THE CILIARY BEAT FREQUENCY OF HUMAN NASAL AND TRACHEAL CILIATED CELLS
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DOI：
10.1016/0248-4900(92)90436-5
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发表时间：
1992-01-01
影响因子：
2.7
通讯作者：
BLAIVE, B
中科院分区：
文献类型：
--
作者：
CLARYMEINESZ, CF;COSSON, J;BLAIVE, B
尽管所有人类的呼吸纤毛在结构上都是相似的，但它们在鼻窝的初始部分（充当热交换器）和气管的下部之间经历的温度范围很大，特别是当我们吸入极冷或极热的空气时。人鼻黏膜和支气管粘膜纤毛细胞在室温下的纤毛搏动频率平均为8hz。在本研究中，我们使用体外和离体两种不同的技术，比较了从5℃到50℃的不同温度下，鼻腔和气管粘膜刷牙中人类细胞的纤毛跳动频率：在体外培养基中取样后不到24小时，在体外根据我们实验室开发的标准程序脱膜和再激活后。测量atp重新激活的纤毛搏动频率使我们能够检查动力蛋白atp酶和所有轴突机械的热参数。在比较两种实验程序的极端温度时，没有观察到鼻窝纤毛和气管纤毛发生频率的显著差异。
Even though all human respiratory cilia are similar in structure, they experience a wide range of temperatures between the initial part of the nasal fossae which behave as heat exchangers and the inferior part of the trachea, particularly when we inhale exceedingly cold or hot air. The ciliary beat frequency of ciliated cells from human nasal mucosa and from bronchial mucosa averages 8 Hz when measured at room temperature. In the present study we compared the ciliary beat frequency of human cells from nasal and tracheal mucosa brushings at different temperatures from 5-degrees-C to 50-degrees-C using two different techniques, ex vivo and in vitro: ex vivo in culture medium less than 24 h after sampling and in vitro after demembranation and reactivation according to a standard procedure developed in our laboratory. Measuring the ATP-reactivated ciliary beat frequency allowed us to check the thermal parameters of the dynein ATPase and all the axonemal machinery. No significant difference in frequency was observed between nasal fossae cilia and tracheal cilia when comparing extreme temperatures in both experimental procedures.