Multiplex analysis of circulating cytokines in the sera of patients with different clinical forms of visceral leishmaniasis
Multiplex analysis of circulating cytokines in the sera of patients with different clinical forms of visceral leishmaniasis
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DOI：
10.1002/cyto.a.20256
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发表时间：
2006-05-01
期刊：
影响因子：
3.7
通讯作者：
Secor, W. Evan
中科院分区：
文献类型：
--
作者：
Kurkjian, Katie M.;Mahmutovic, Aidaj.;Secor, W. Evan
背景：内脏利什曼病(VL)是一种慢性细胞内寄生虫病，其临床范围从亚临床、无症状感染到严重的临床疾病(黑热病)。在实验性利什曼病中，对感染有Th1反应的小鼠往往疾病有限，而Th2反应与疾病进展有关。VL患者的反应通常是混合的，而不是两极分化的。然而，大多数临床研究使用的方法需要相对较大的样本量，因此限制了其范围。使用多重微球分析(MMA)检测血液样本中的多种细胞因子水平可能有助于进一步评估人类的Th1/Th2范式。方法：居住在NFL流行区的孟加拉人(n=120)被分为五个临床类别之一。采用复合微球细胞因子免疫分析法检测患者血清中IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IIA2、干扰素-γ和肿瘤坏死因子-α的水平。黑热病患者IL-8、IL-10水平最高，而IL-12中值较低。结论：细胞因子MMA是一种非常及时、有效的检测内脏血吸虫病患者循环细胞因子水平的方法。(C)2006年国际学会kw分析细胞学
Background: The clinical spectrum of visceral leishmaniasis (VL), a chronic intracellular parasitic disease, ranges from a subclinical, asymptomatic infection to severe clinical disease (kala-azar). In experimental leishmaniasis, mice that have a Th1 response to infection tend to have limited disease while a Th2 response is associated with disease progression. Humans with VL most often have mixed rather than polarized responses. However, most clinical studies have used methods that require a relatively large sample volume, thus limiting their scope. Measuring multiple cytokine levels in blood samples using a multiplexed microsphere assay (MMA) may be useful to further evaluate the Th1/Th2 paradigm in humans.Methods: Bangladeshi individuals (n = 120) living in in area endemic for NFL were categorized into one of the five clinical categories. Sera from these individuals were measured for levels of IL2, IL-4, IL-5, IL-6, IL-8, IL-10, IIA2, IFN-gamma, and TNF-alpha by multiplexed microsphere cytokine immunoassay.Results: Circulating IL-8, IL-10, and IL-12 differed significantly among the clinical groups. Persons with kala-azar lem on stra ted the highest niedian levels of IL-8 and IL-10 but lower median levels of IL-12.Conclusions: The MMA for cytokines is an extremely timeand sample-efficicrit method for characterizing cirCUlating cytokine levels in visceral lcishrnaniasis patients. (c) 2006 International Society kw Analytical Cytology