A panel of eight autophagy-related long non-coding RNAs is a good predictive parameter for clear cell renal cell carcinoma
A panel of eight autophagy-related long non-coding RNAs is a good predictive parameter for clear cell renal cell carcinoma
复制标题
一组八种与自噬相关的长非编码 RNA 是透明细胞肾细胞癌的良好预测参数
DOI：
10.1016/j.ygeno.2021.01.016
复制
发表时间：
2021-02-05
期刊：
影响因子：
4.4
通讯作者：
Luo, Jun-Hang
中科院分区：
文献类型：
--
作者：
Gui, Cheng-Peng;Cao, Jia-Zheng;Luo, Jun-Hang
透明细胞肾细胞癌（ccRCC）预后不定。预后生物标志物可以根据风险对患者进行分层，并为临床决策提供关键信息。我们筛选了自噬相关的长非编码lncRNA（lncRNA）签名，以改善癌症基因组图谱（TCGA）数据库中的术后风险分层。我们在ICGC和SYSU队列中证实了该模型是一个重要的独立预后标志。用Western blotting、自噬流测定和透射电镜观察证实了8种与自噬相关的lncRNA的表达调控影响了体外自噬流的变化。我们的数据表明，与自噬相关的8- 1 ncRNA标签是预测ccRCC患者生存的有希望的预后工具。将该特征与临床和病理参数相结合可以帮助准确的风险评估以指导临床管理，并且与自噬相关的8-lncRNA特征可以作为治疗靶点。
Clear-cell renal cell carcinoma (ccRCC) carries a variable prognosis. Prognostic biomarkers can stratify patients according to risk, and can provide crucial information for clinical decision-making. We screened for an autophagy-related long non-coding lncRNA (lncRNA) signature to improve postoperative risk stratification in The Cancer Genome Atlas (TCGA) database. We confirmed this model in ICGC and SYSU cohorts as a significant and independent prognostic signature. Western blotting, autophagic-flux assay and transmission electron microscopy were used to verify that regulation of expression of 8 lncRNAs related to autophagy affected changes in autophagic flow in vitro. Our data suggest that 8-1ncRNA signature related to autophagy is a promising prognostic tool in predicting the survival of patients with ccRCC. Combination of this signature with clinical and pathologic parameters could aid accurate risk assessment to guide clinical management, and this 8-lncRNAs signature related to autophagy may serve as a therapeutic target.