High-resolution surface plasmon resonance sensors based on a dove prism
High-resolution surface plasmon resonance sensors based on a dove prism
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DOI：
10.1016/j.talanta.2008.10.006
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发表时间：
2009-03-15
期刊：
影响因子：
6.1
通讯作者：
Masson, Jean-Francois
中科院分区：
文献类型：
--
作者：
Bolduc, Olivier R.;Live, Ludovic S.;Masson, Jean-Francois
使用道威棱镜的波长询问表面等离子体共振（SPR）光谱将简单且廉价的光学设计与高分辨率折射率监测和生物传感相结合。BK 7道威棱镜以72.8度的全内反射角（SPR中有效的角度）反转光学图像。因此，一个独特的系统可以实现SPR生物传感使用波长询问，也执行SPR成像。这种光学配置有利地在每个光学部件之间使用单轴光路，简化了SPR仪器的光学设计，而不损害分析性能。射流也被纳入仪器设计，以有效的样品输送。SPR仪器的特点是在折射率（RI）的灵敏度，RI分辨率，再现性，和应用程序监测低浓度的生物事件。数据分析方法进行了比较，以提高测量响应的分辨率。使用最小搜索程序分析的原始数据导致RI分辨率在10（-6）范围内，而用奇异值分解预处理数据将分辨率提高一个数量级。根据所采用的分光光度计，可以很容易地调整可达到的RI范围：显示了550-850 nm和550-1100 nm波长计的示例，结果分别在1.32-1.39 RIU和1.32-1.42 RIU的RI范围内。使用生物传感器的波长询问配置进行β-内酰胺酶μ M浓度的监测。最后，使用具有带通滤波器和CCD相机的道威棱镜SPR获得具有水滴（体积= 500 nL）的表面的SPR图像。使用道威棱镜配置的SPR在一个独特的平台上结合了便携式SPR仪器、SPR成像仪和研究级SPR仪器的优点。(C)2008 Elsevier B. V.保留所有权利。
Wavelength interrogation surface plasmon resonance (SPR) spectroscopy using a dove prism combines a simple and inexpensive optical design with high-resolution refractive index monitoring and biosensing. A BK7 dove prism inverts an optical image with a total internal reflection angle of 72.8 degrees, an angle active in SPR. Hence, a unique system can accomplish SPR biosensing using wavelength interrogation and also perform SPR imaging. This optical configuration advantageously uses a single axis optical path between each optical component, simplifying the optical design of SPR instruments without compromise of the analytical performance. Fluidics were also incorporated to the instrument design for efficient sample delivery. The SPR instrument is characterized in terms of refractive index (RI) sensitivity, RI resolution, reproducibility, and application for monitoring low concentration biological events. Data analysis methodologies are compared for improved resolution of the measured response. Raw data analyzed using a minimum hunting procedure results in RI resolution in the 10(-6) range, while pre-treating data with singular value decomposition improves the resolution by one order of magnitude. Depending on the spectrophotometer employed, the RI range accessible can be easily tuned: examples with a 550-850 nm and a 550-1100 nm spectrophotometers are shown and results respectively in RI ranges of 1.32-1.39 RIU and 1.32-1.42 RIU. Monitoring of mu M concentration of beta-lactamase is performed using the wavelength interrogation configuration of the biosensor. Finally, a SPR image of a surface with a water droplet (volume = 500 nL) was obtained using the dove prism SPR with a band pass filter and a CCD camera. SPR using a dove prism configuration combines advantages of portable SPR instruments, SPR imagers and research-grade SPR instruments in a unique platform. (C) 2008 Elsevier B.V. All rights reserved.