Associations of Serum Cytokine Levels and Interleukin-6-572C/G Polymorphism with Myelin Damage in Chinese Children with Autism Spectrum Disorder
Associations of Serum Cytokine Levels and Interleukin-6-572C/G Polymorphism with Myelin Damage in Chinese Children with Autism Spectrum Disorder
复制标题
中国自闭症谱系障碍儿童血清细胞因子水平和白介素6-572C/G多态性与髓磷脂损伤的关系
DOI：
10.1016/j.neuroscience.2021.04.006
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发表时间：
2021-05-11
期刊：
影响因子：
3.3
通讯作者：
Zhang, Xin
中科院分区：
文献类型：
--
作者：
Han, Yu;Xiong, Wenjuan;Zhang, Xin
越来越多的证据表明，免疫紊乱和轴突髓鞘形成异常参与自闭症谱系障碍（ASD）的病理生理。本研究旨在确定细胞因子在中国ASD儿童髓鞘损伤中的作用，以及细胞因子失调、髓鞘损伤和细胞因子多态性在中国ASD儿童中的作用。本病例对照研究包括98例ASD受试者和252例典型发育（TD）对照;血清细胞因子和髓鞘碱性蛋白（MBP）水平采用酶联免疫吸附测定法测定。采用聚合酶链反应-限制性片段长度多态性分析对细胞因子多态性进行基因分型。自闭症的临床表现进行了评估，儿童自闭症量表（汽车）。结果表明，ASD患儿血清IL-1 β、IL-2 R、IL-6、IL-8和MBP水平均高于TD患儿。在ASD患者中，血清MBP水平与汽车总分显著正相关，血清IL-1 β、IL-2 R、IL-6和MBP水平呈正相关。IL-6*MBP是影响ASD发病风险的因素，IL-2 R *MBP是影响ASD症状严重程度的因素，影响ASD的辅助诊断。白细胞介素-6-572CC基因型的存在与血清IL-6和MBP水平显著升高相关，但不影响ASD的风险和症状严重程度。提示ASD患儿存在炎症反应和髓鞘损伤。细胞因子失调影响ASD中的髓鞘损伤;此外，细胞因子和髓鞘损伤的相互作用影响ASD的风险和症状严重程度。IL-6- 572 C/G基因型可能通过影响IL-6的循环水平而与ASD的髓鞘损伤有关。(C)2021年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Increasing evidence suggests that immunological disturbances and abnormalities in axonal myelination are involved in the pathophysiology of autism spectrum disorder (ASD). The present study aimed to determine the role of cytokines in myelin damage in Chinese children with ASD and the role of cytokine dysregulation, myelin damage, and cytokine polymorphisms in ASD in Chinese children. The present case-control study included 98 ASD subjects and 252 typically developing (TD) controls; the levels of serum cytokines and myelin basic protein (MBP) were determined using enzyme-linked immunosorbent assay. Cytokine polymorphisms were genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis. Autistic clinical manifestations were assessed by the Childhood Autism Rating Scale (CARS). The results showed that serum levels of interleukin (IL)-1 beta, IL-2R, IL-6, IL-8, and MBP were higher in children with ASD compared with those in TD children. In individuals with ASD, serum MBP level was significantly positively associated with the CARS total score, and serum levels of IL-1 beta, IL-2R, IL-6, and MBP demonstrated positive correlations. The data identified IL-6*MBP as a factor that influenced the risk of ASD, and IL-2R*MBP was identified as a factor that influenced symptom severity, which influenced auxiliary diagnosis of ASD. The presence of the interleukin-6-572CC genotype was associated with significantly higher serum levels of IL-6 and MBP but did not influence the risk and symptom severity of ASD. Therefore, the results suggested inflammatory responses and myelin damage in Chinese children with ASD. Cytokine dysregulation influenced myelin damage in ASD; moreover, the interactions of the cytokines and myelin damage influenced the risk and symptom severity of ASD. The IL-6-572C/G genotypes may be associated with myelin damage in ASD by influencing the circulating level of IL 6. (C) 2021 IBRO. Published by Elsevier Ltd. All rights reserved.