Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
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DOI：
10.1002/etc.3362
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发表时间：
2016-08-01
影响因子：
4.1
通讯作者：
Xu, Lei
中科院分区：
文献类型：
--
作者：
Liu, Lili;Yan, Yanchun;Xu, Lei
Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal-sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1-cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose- and time-dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. Environ Toxicol Chem 2016;35:2066-2073. (c) 2016 SETAC
