Early and late paternal effects of reactive oxygen species in semen on embryo development after intracytoplasmic sperm injection
Early and late paternal effects of reactive oxygen species in semen on embryo development after intracytoplasmic sperm injection
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DOI：
10.1080/19396368.2020.1720865
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发表时间：
2020-02-10
影响因子：
2.4
通讯作者：
Yumura, Yasushi
中科院分区：
文献类型：
--
作者：
Kuroda, Shinnosuke;Takeshima, Teppei;Yumura, Yasushi
虽然精液中的活性氧与辅助生殖技术的不利结果有关，但它们对胚胎发育详细阶段的影响尚不清楚。我们研究了精液中活性氧与卵母细胞受精率、卵裂率和囊胚形成率的关系。这项回顾性研究纳入了77对接受卵胞浆内单精子注射的夫妇，并分析了141个卵胞浆内单精子注射周期的887个卵子。比较受精组与未受精组、卵裂良好胚胎组与未发育胚胎组、优质囊胚组与劣质囊胚组精液中活性氧水平。计算活性氧的临界水平，预测良好卵裂胚胎和优质囊胚发育。受精率为65.4%，平均活性氧水平之间的受精和非受精组没有显着差异。未发育胚胎组活性氧水平显著高于卵裂良好胚胎组（P = 0.026），优质囊胚组活性氧水平显著低于劣质胚胎组（P = 0.015）。卵裂胚胎和囊胚分为高和低活性氧组使用的截止值为6601和4926相对光单位，从接收器工作特性曲线计算。高活性氧组卵裂良好胚胎率和优质囊胚率均低于低活性氧组，差异均有统计学意义。总之，精液中的活性氧被认为对卵胞浆内单精子注射中胚胎发育的早期和晚期都有不利影响。
Although reactive oxygen species in semen are associated with unfavorable results with respect to assisted reproductive technology, their effects based on the detailed stages of embryo development are unclear. We investigated the relationship between reactive oxygen species in semen and the oocyte fertilization rate, cleavage rate, and blastulation rate of intracytoplasmic sperm injections. This retrospective study enrolled 77 couples who underwent intracytoplasmic sperm injection and analyzed 887 eggs from 141 cycles of intracytoplasmic sperm injection. The reactive oxygen species level in semen was compared between the fertilized and nonfertilized groups, between the good-cleavage-embryo and non-developed-embryo groups, and between the good-quality-blastocyst and poor-quality-blastocyst groups. The cut-off level of reactive oxygen species was calculated to predict good-cleavage-embryo and good-quality-blastocyst development. The fertilization rate was 65.4%, and the mean reactive oxygen species levels were not significantly different between the fertilized and nonfertilized groups. The reactive oxygen species level was significantly higher in the non-developed-embryo group than in the good-cleavage-embryo group (P = 0.026) and was significantly lower in the good-quality-blastocyst group than in the poor-quality-embryo group (P = 0.015). Cleavage embryos and blastocysts were divided into high- and low-reactive-oxygen-species groups using a cut-off value of 6601 and 4926 relative light units, as calculated from the receiver operating characteristic curve. The rates of good-cleavage embryos and good-quality blastocysts were lower in the high-reactive-oxygen-species group than in the low-reactive-oxygen-species group, which were both statistically significant. To conclude, reactive oxygen species in semen is considered to have an adverse effect on both the early and late stages of embryo development in intracytoplasmic sperm injection.