Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis
Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis
复制标题
DOI：
10.1364/boe.7.001511
复制
发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
Boccara, A. Claude
中科院分区：
文献类型：
--
作者：
Apelian, Clement;Harms, Fabrice;Boccara, A. Claude
我们开发了一种新的内源性方法来揭示新鲜离体组织中的亚细胞代谢对比度，利用全场光学相干断层扫描干涉信号的时间依赖性。该方法揭示了与内源性散射元件的局部活性相关的信号，这可以揭示其他基于OCT的技术失败或需要外源性造影剂的细胞。我们受益于全视野OCT的微米横向分辨率来成像细胞内特征。我们使用这种时间依赖性来识别正常或病理条件下广泛器官上毫秒级的不同动态。(C)2016美国光学学会
We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions. (C) 2016 Optical Society of America