Grid-Layout and Theta-Modulation of Layer 2 Pyramidal Neurons in Medial Entorhinal Cortex
Grid-Layout and Theta-Modulation of Layer 2 Pyramidal Neurons in Medial Entorhinal Cortex
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DOI：
10.1126/science.1243028
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发表时间：
2014-02-21
期刊：
影响因子：
56.9
通讯作者：
Brecht, Michael
中科院分区：
文献类型：
--
作者：
Ray, Saikat;Naumann, Robert;Brecht, Michael
关于内侧内嗅皮层第2层的微电路是如何组织的，我们知之甚少。我们可视化的主要细胞微电路，并确定在自由活动的大鼠细胞θ-节律。非齿状突起，钙结合蛋白阳性的锥体细胞束树突在一起，形成补丁排列在一个六边形网格对齐层1轴突，subsciculum，和胆碱能输入。钙结合蛋白阴性，齿状回突出的星状细胞分布在整个层2，但避免中心的钙结合蛋白阳性补丁。胆碱能驱动持续θ-节律，这是两倍强于星状神经元的锥体。θ-节律性是细胞类型特异性的，但不像细胞内在特性所预期的那样分布。第2层分为一个弱θ锁定的星状细胞晶格和时空高度组织的金字塔网格。需要评估这两个不同的主要细胞网络如何有助于网格细胞的活动。
Little is known about how microcircuits are organized in layer 2 of the medial entorhinal cortex. We visualized principal cell microcircuits and determined cellular theta-rhythmicity in freely moving rats. Non-dentate-projecting, calbindin-positive pyramidal cells bundled dendrites together and formed patches arranged in a hexagonal grid aligned to layer 1 axons, parasubiculum, and cholinergic inputs. Calbindin-negative, dentate-gyrus-projecting stellate cells were distributed across layer 2 but avoided centers of calbindin-positive patches. Cholinergic drive sustained theta-rhythmicity, which was twofold stronger in pyramidal than in stellate neurons. Theta-rhythmicity was cell-type-specific but not distributed as expected from cell-intrinsic properties. Layer 2 divides into a weakly theta-locked stellate cell lattice and spatiotemporally highly organized pyramidal grid. It needs to be assessed how these two distinct principal cell networks contribute to grid cell activity.