Mechanically Drawn Hydrogels Uniaxially Orient Hydroxyapatite Crystals and Cell Extension
Mechanically Drawn Hydrogels Uniaxially Orient Hydroxyapatite Crystals and Cell Extension
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DOI：
10.1021/cm0490975
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发表时间：
2004-12
影响因子：
8.6
通讯作者：
T. Kaneko;D. Ogomi;Ryoko Mitsugi;T. Serizawa;M. Akashi
中科院分区：
文献类型：
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作者：
T. Kaneko;D. Ogomi;Ryoko Mitsugi;T. Serizawa;M. Akashi
冻融聚乙烯醇水凝胶通过单轴机械拉伸至其原始长度的1 - 5倍进行结构取向，并通过使用戊二醛（20 mol %）的连续化学交联固定取向。通过磷酸盐和钙离子的交替浸泡，成功地将羟基磷灰石（HAp）晶体矿化到定向水凝胶上。X-射线衍射图像证实HAp晶胞的c-轴垂直于水凝胶的表面取向。此外，偏振红外光谱证实了磷酸根离子的取向。我们研究了这些结构取向的HAp-PVA水凝胶杂化物对细胞伸展各向异性的影响。扫描电子显微镜证实小鼠成纤维细胞（L929）沿着拉伸方向延伸到定向的HAp−水凝胶杂交体上。这些结果可能会导致发展的组织工程支架产生定向骨组织。
Freeze−thawed poly(vinyl alcohol) hydrogels were structurally oriented by uniaxial mechanical drawing up to 1−5 times their original length, and the orientation was fixed by successive chemical cross-linking using glutaraldehyde (20 mol %). Hydroxyapatite (HAp) crystals were successfully mineralized onto the oriented hydrogels by repeated alternate immersion into the solutions of phosphate ion and calcium ion. X-ray diffraction images confirmed that the c-axis of the HAp crystal cell was oriented perpendicularly to the surface of the hydrogel. In addition, polarized infrared spectroscopy confirmed the orientation of the phosphate ion. We investigated the effects of these structurally oriented HAp−PVA hydrogel hybrids on the anisotropy of cell extension. Scanning electron microscopy confirmed that mouse fibroblasts (L929) extended onto the oriented HAp−hydrogel hybrid along the drawing direction. These results may lead to the development of a tissue engineering scaffold for yielding oriented bony tissues.