Molecular cloning and sequence analysis of the cDNA for rat mitochondrial enoyl-CoA hydratase
Molecular cloning and sequence analysis of the cDNA for rat mitochondrial enoyl-CoA hydratase
复制标题
大鼠线粒体烯酰辅酶A水合酶cDNA的分子克隆和序列分析
DOI：
10.1111/j.1432-1033.1989.tb15083.x
复制
发表时间：
1989
期刊：
影响因子：
5.4
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
--
作者：
N. Minami;S. Taketani;T. Osumi;T. Hashimoto
为了阐明β-氧化系统的线粒体和过氧化物酶体同工酶之间的结构关系，对线粒体烯酰辅酶A水合酶的cDNA进行了克隆和测序。 1454 bp的cDNA序列包含870 bp的开放阅读框，编码290个氨基酸残基的多肽。与成熟酶的氨基末端序列相比，预测的序列在氨基末端含有29个残基的前序列。该前序列具有线粒体信号肽的典型特征。该酶的一级结构与过氧化物酶体烯酰辅酶A水合酶：3-羟酰辅酶A脱氢酶双功能酶的氨基末端部分序列显着相似。后一种酶的羧基末端部分与线粒体 3-羟酰基-CoA 脱氢酶具有序列相似性 [Ishii, N., Hijikata, M., Osumi, T. & Hashimoto, T. (1987) J. Biol.化学。 262, 8144–8150]。这些发现表明过氧化物酶体双功能酶分别在氨基端和羧基端侧具有水合酶和脱氢酶功能。线粒体β-氧化酶和过氧化物酶体双功能酶可能具有共同的进化起源。
To elucidate structural relationships between the mitochondrial and peroxisomal isozymes of β-oxidation systems, cDNA of the mitochondrial enoyl-CoA hydratase was cloned and sequenced. The 1454-bp cDNA sequence contained a 870 bp of open reading frame, encoding a polypeptide of 290 amino acid residues. When compared with the amino-terminal sequence of the mature enzyme, the predicted sequence contained a 29-residue presequence at the amino terminus. This presequence had characteristics typical of a mitochondrial signal peptide. The primary structure of this enzyme showed significant similarity with the amino-terminal portion of sequence of the peroxisomal enoyl-CoA hydratase: 3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme. The carboxy-terminal part of the latter enzyme has sequence similarity with mitochondrial 3-hydroxyacyl-CoA dehydrogenase [Ishii, N., Hijikata, M., Osumi, T. & Hashimoto, T. (1987) J. Biol. Chem. 262, 8144–8150]. These findings suggest that the peroxisomal bifunctional enzyme has the hydratase and dehydrogenase functions on the amino- and carboxy-terminal sides, respectively. The mitochondrial β-oxidation enzymes and the peroxisomal bifunctional enzyme may have common evolutionary origins.
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW