Low Antioxidant Enzyme Levels and Oxidative Stress in Laryngopharyngeal Reflux (LPR) Patients
Low Antioxidant Enzyme Levels and Oxidative Stress in Laryngopharyngeal Reflux (LPR) Patients
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DOI：
10.1016/j.jvoice.2021.05.018
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发表时间：
2023-11-03
期刊：
影响因子：
2.2
通讯作者：
Ballica, Basak
中科院分区：
文献类型：
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作者：
Bulut, Fuat;Tetiker, Aylin Turksever;Ballica, Basak
目的：喉咽反流病（LPR）的症状与胃食管反流病（GERD）不同。 LPR 可引起慢性粘膜炎症，从而导致细胞因子产生增加和抗氧化酶水平全身降低。本研究的目的是评估LPR患者的抗氧化酶水平。方法：对因典型LPR主诉而到耳鼻喉科门诊就诊的患者进行反流症状指数（RSI）问卷、纳入RSI的食管外症状问卷以及70度硬性喉镜反流发现评分（RFS）评估，其中RSI评分在13以上且RFS评分在7以上的患者60例纳入研究研究。对照组包括三十名健康志愿者。测定患者血清中抗氧化酶SOD、GSH-Px、CAT水平，并与对照组进行比较。生化测试获得的结果表示为平均值+/-SE。使用描述性统计方法（平均值+/-标准误差）对对照组和研究组进行独立t检验。 P < 0.05为差异有统计学意义。结果：LPR组中，女性28例（46%），男性32例（53%），年龄范围为21-60岁，平均年龄为36.45±1.147。LPR组与对照组在年龄、性别和体重指数（BMI）方面无显着差异。在LPR组中，RSI最低得分为14，最高得分为39。平均RSI为23.67。 RFS 范围为 8-22。平均 RFS 为 13.50。 RSI 和总 RFS 之间观察到高度显着的统计相关性 (P < 0.001)。对照组和LPR组的抗氧化酶水平存在显着差异。对照组抗氧化酶水平结果为SOD 274.10±26.836 U/L、GSH-Px 174.20±20.699 mu U/mL、CAT 42.2898±20.699 KU/L。LPR组抗氧化酶水平结果为SOD 147±14.022 U/L(P < 0.01)、GSH-Px 88.28 +/- 9.113 U/mL (P < 0.01) 和 CAT 12.67 +/- 0.799 KU/L (P < 0.001)。 RSI 结果范围为 4 至 39，RFS 范围为 8 至 22。抗氧化酶水平与 RFS 和 RFS 的各个变量表现出相当一致的可靠性。 RSI和RFS之间也存在高度显着的统计相关性。结论：我们发现LPR患者的抗氧化酶SOD、GPX和过氧化氢酶水平显着降低。应研究减少 LPR 氧化应激 (OS) 的治疗方式。
Objective: Laryngopharyngeal reflux disease (LPR) is a characterized by symptoms different from gastroesophageal reflux disease (GERD). LPR can causes chronic mucosal inflammation which may lead to an increase in cytokine production, and a systemic decrease in antioxidant enzyme levels. Our aim in this study is to evaluate antioxidant enzyme levels in patients with LPR.Methods: Reflux Symptom Index (RSI) questionnaire, extraesophageal symptom questionnaire which is included in RSI and Reflux Finding Score (RFS) evaluation with 70 degrees rigid laryngoscope were performed to patients who applied to the otolaryngology clinic with a typical LPR complaint, and 60 patients who had an RSI score above 13 and an RFS score above 7 were included in the study. Thirty people consisting of healthy volunteers were included in the control group. Antioxidant enzyme SOD, GSH-Px and CAT levels were measured in the blood serum of the patients and compared with the control group. Results obtained from biochemical tests were expressed as mean +/- SE. Descriptive statistical methods (mean +/- standard error) were used for the independent t test for the control and study group. P < 0.05 was considered statistically significant.Results: In the LPR group, 28 (46%) were women, 32 (53%) were men, and age range was 21-60, average age was 36.45 +/- 1.147.There was no significant difference between LPR and control group in terms of age, gender and Body Mass Index (BMI). In the LPR group, the lowest score for RSI was 14 and the highest score was 39. The average RSI was 23.67. RFS ranges from 8-22. The mean RFS was 13.50. A highly significant statistical correlation was observed between RSI and total RFS (P < 0.001). There was a significant difference between the antioxidant enzyme levels of the control group and the LPR group. Antioxidant enzyme levels of the control group were SOD 274.10 +/- 26.836 U / L, GSH-Px 174.20 +/- 20.699 mu U / mL and CAT 42.2898 +/- 20.699 KU / L. Antioxidant enzyme level results of the LPR group were SOD 147 +/- 14.022 U / L (P < 0.01), GSH-Px 88.28 +/- 9.113 U / mL (P < 0.01) and CAT 12.67 +/- 0.799 KU / L (P < 0.001). The RSI results ranges from 4 to 39 and the RFS from 8 to 22. Antioxidant enzyme levels demonstrated fairly consistent reliability with individual variables from both RFS and RFS. There was also a highly significant statistical correlation between RSI and RFS.Conclusion: We found that the antioxidant enzymes SOD, GPX and catalase enzyme levels were significantly lower in LPR patients. Treatment modalities to reduce oxidative stress (OS) in LPR should be investigated.