Mobilicoccus pelagius gen. nov., sp nov and Piscicoccus intestinalis gen. nov, sp nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.
Mobilicoccus pelagius gen. nov., sp nov and Piscicoccus intestinalis gen. nov, sp nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.
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DOI：
10.2323/jgam.56.427
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发表时间：
2010-12-01
影响因子：
1.2
通讯作者：
Suzuki, Ken-ichiro
中科院分区：
文献类型：
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作者：
Hamada, Moriyuki;Iino, Takao;Suzuki, Ken-ichiro
从鱼类肠道中分离到革兰氏阳性菌Aji5-31(T)和Ngc37-23(T)。16S rRNA基因序列分析表明，两株菌株与皮肤菌科成员有亲缘关系，16S rRNA基因序列相似性为95.6 ~ 96.9%。皮嗜菌科包括2属3种：刚果皮嗜菌、chelon皮嗜菌和limosa皮嗜菌。然而，由于尚不清楚D. chelonae的化学分类特征，因此有人建议使用多相方法重新研究其分类位置（Stackebrandt, 2006; Stackebrandt and Schumann, 2000）。本研究表明，菌株aj5 -31(T)、Ngc37-23(T)和D. chelonae NBRC 105200(T)应与其他皮肤菌科成员分离，因为菌株aj5 -31(T)的甲基萘醌主要成分为MK-8(H-2)，菌株Ngc37-23(T)以异支脂肪酸为主要成分，D. chelonae的甲基萘醌组成为MK-8（H-4）、MK-8和MK-8(H-2)（分别为5:3:2）。基于这些显著的表型特征和系统发育分析结果，我们建议将菌株aj5 -31(T)和Ngc37-23(T)划分为皮肤菌科的两个新属和新种。命名为pelagius Mobilicoccus gen. nov., sp. nov.和Piscicoccus ntestinalis gen. nov., sp. nov.，类型菌株分别为aj5 -31(T) (=NBRC 104925（T) = DSM 22762(T)）和Ngc37-23(T) (=NBRC 104926（T) = DSM 22761(T)）。此外，螯龙应重新划归为皮癣科新属，命名为螯龙Austwickia chelonae gen. nov., comb。11月。
Two Gram-positive bacteria, designated strains Aji5-31(T) and Ngc37-23(T), were isolated from the intestinal tracts of fishes. 16S rRNA gene sequence analysis indicated that both strains were related to the members of the family Dermatophilaceae, with 95.6-96.9% 16S rRNA gene sequence similarities. The family Dermatophilaceae contains 2 genera and 3 species: Dermatophilus congolensis, Dermatophilus chelonae and Kineosphaera limosa. However, it has been suggested that the taxonomic position of D. chelonae should be reinvestigated using a polyphasic approach, because the chemotaxonomic characteristics are not known (Stackebrandt, 2006; Stackebrandt and Schumann, 2000). Our present study revealed that strains Aji5-31(T), Ngc37-23(T) and D. chelonae NBRC 105200(T) should be separated from the other members of the family Dermatophilaceae on the basis of the following characteristics: the predominant menaquinone of strain Aji5-31(T) is MK-8(H-2), strain Ngc37-23(T) possesses iso- branched fatty acids as major components, and the menaquinone composition of D. chelonae is MK-8(H-4), MK-8 and MK-8(H-2) (5 : 3 : 2, respectively). On the basis of these distinctive phenotypic characteristics and phylogenetic analysis results, it is proposed that strains Aji5-31(T) and Ngc37-23(T) be classified as two novel genera and species of the family Dermatophilaceae. The names are Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., and the type strains are Aji5-31(T) (=NBRC 104925(T) = DSM 22762(T)) and Ngc37-23(T) (=NBRC 104926(T) = DSM 22761(T)), respectively. In addition, D. chelonae should be reassigned to a new genus of the family Dermatophilaceae with the name Austwickia chelonae gen. nov., comb. nov.