The large external domain is sufficient for the correct sorting of secreted or chimeric influenza virus hemagglutinins in polarized monkey kidney cells.
The large external domain is sufficient for the correct sorting of secreted or chimeric influenza virus hemagglutinins in polarized monkey kidney cells.
复制标题
大的外部结构域足以正确分选极化猴肾细胞中分泌的或嵌合的流感病毒血凝素。
DOI：
10.1083/jcb.104.3.769
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Rodriguez-Boulan,E
中科院分区：
文献类型：
--
作者：
Roth,MG;Gundersen,D;Patil,N;Rodriguez-Boulan,E
MA104。11恒河猴肾细胞表达极化上皮细胞的几个特征，包括在不可渗透基质上形成”圆顶”，在胶原凝胶上生长时建立跨单层电阻，流感和水疱性口炎病毒的极化成熟，以及这些病毒的糖蛋白在单个表面结构域的表达。在MA 104中维持流感病毒血凝素（HA）的极化表达。用SV 40衍生的载体感染细胞，所述载体携带野生型流感病毒HA的cDNA基因、编码分泌型HA（HA~ c）的截短的HA基因、或编码具有HA的外部结构域和水疱性口炎病毒G蛋白（HAG）的跨膜和胞质结构域的杂合蛋白的嵌合基因。因此，将预定用于顶表面的糖蛋白（例如HA）与预定用于基底外侧膜的蛋白质（例如G）分离的识别事件涉及蛋白质的外部结构域的特征。透明质酸的跨膜区和胞质区在此过程中不起作用。UKARVOTIC细胞引导蛋白质从共同的合成位点向不同的最终目的地进行复杂的内部运输。在极化的上皮细胞中，这种运输的一个方面是将分泌的和膜结合的蛋白质运输到质膜的结构上不同的结构域。这些表面结构域由闭锁小带的连接复合物界定，每个结构域都含有其功能特化所需的独特蛋白质组（Rodriguez-Boulan，1983 a; Simons和Fuller，1985）。显然，这些蛋白质组的识别、分选和定向运输是有机制的。
MA104. 11 rhesus kidney cells express several characteristics of polarized epithelial cells, including the formation of" domes" on impermeable substrates, the establishment of a transmonolayer electrical resistance when grown on collagen gels, the polarized maturation of influenza and vesicular stomatitis viruses, and the expression of the glycoproteins of those viruses at a single surface domain. The polarized expression of the influenza virus hemagglutinin (HA) is maintained in MA104. 11 cells infected with SV40-derived vectors carrying a cDNA gene for either the wild-type influenza virus HA, a truncated HA gene encoding a secreted form of HA (HA~ c), or a chimeric gene encoding a hybrid protein with the external domain of the HA and the transmembrane and cytoplasmic domains of the vesicular stomatitis virus G protein (HAG). Thus, the recognition event separating glycoproteins, such as HA, destined for the apical surface from proteins, such as G, destined for the basolateral membranes involves features of the external domains of the proteins. The transmembrane and cytoplasmic domains of HA have no role in this process.UKARVOTIC cells direct a complex internal traffic of proteins moving from common sites of synthesis to separate final destinations. In polarized epithelial cells an aspect of this traffic is the transport of secreted and membrane-bound proteins to structurally distinct domains of the plasmalemma. These surface domains, delimited by the junctional complexes of the zonula occludentes, each contain unique sets of proteins required for their functional specialization (reviewed by Rodriguez-Boulan, 1983a; Simons and Fuller, 1985). Obviously, there are mechanisms for the recognition, sorting, and directional transport of these sets of proteins.