ASSOCIATION OF ESCHERICHIA-COLI HEP-2 ADHERENCE PATTERNS WITH TYPE AND DURATION OF DIARRHEA
ASSOCIATION OF ESCHERICHIA-COLI HEP-2 ADHERENCE PATTERNS WITH TYPE AND DURATION OF DIARRHEA
复制标题
DOI：
10.1016/0140-6736(91)90868-p
复制
发表时间：
1991-02-02
期刊：
影响因子：
168.9
通讯作者：
ESLAVA, C
中科院分区：
文献类型：
--
作者：
CRAVIOTO, A;TELLO, A;ESLAVA, C
72名墨西哥儿童出生后头两年的636份粪便标本中，有373份(59%)检出粘附性大肠杆菌(Hep-2细胞)。具有局部粘附性的菌株与急性非血性腹泻显著相关，而具有聚集性粘附性的菌株与持续性腹泻显著相关。有局部粘连的菌株中有一半不是肠源性大肠埃希氏菌血清型，也没有与肠道致病性大肠埃希氏菌黏附因子DNA探针杂交。所有有局部粘连的菌株都进行了荧光肌动蛋白染色(Fas)检测，与血清型无关。被聚集性菌株定居的儿童中有三分之一患有血性腹泻。分离具有弥漫粘附性的菌株与腹泻的类型或持续时间无关，但通常与另一种致病微生物的分离有关。
373 (59%) out of 636 faecal specimens obtained during the first 2 years of life of 72 Mexican children yielded adherent Escherichia coli (HEp-2 cells). Strains with localised adherence were significantly associated with acute non-bloody diarrhoea, whereas strains with aggregative adherence were significantly associated with persistent diarrhoea. Half the strains with localised adherence were not enteropathogenic E coli serotypes nor did they hybridise with an enteropathogenic E coli adherence factor DNA probe. All strains with localised adherence gave a positive fluorescent actin staining (FAS) assay, irrespective of serotype. One-third of children colonised by aggregative strains had bloody diarrhoea. Isolation of strains with diffuse adherence was not related to type or duration of diarrhoea but was generally associated with isolation of another pathogenic organism.