Enrichment of marine manganese-oxidizing microorganisms using polycaprolactone as a solid organic substrate
Enrichment of marine manganese-oxidizing microorganisms using polycaprolactone as a solid organic substrate
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DOI：
10.1007/s10529-021-03088-z
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发表时间：
2021-01-26
影响因子：
2.7
通讯作者：
Yamaguchi, Takashi
中科院分区：
文献类型：
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作者：
Aoki, Masataka;Miyashita, Yukina;Yamaguchi, Takashi
目的 负责生物氧化锰 (Bio-MnOx) 生产的异养锰 (Mn) 氧化微生物非常挑剔。仅通过向非无菌混合培养物中添加可溶性有机底物并不容易实现它们的富集。本研究的目的是评估聚己内酯（PCL）（一种脂肪族聚酯）作为富集海洋锰氧化微生物的有效固体有机底物。结果我们使用 PCL 作为固体有机基质，成功获得了具有溶解锰去除和 MnOx 生产能力的海洋微生物富集。锰氧化富集培养物去除溶解锰遵循一级动力学，速率常数为0.014 h(-1)。 16S rRNA 基因扩增子测序分析表明，锰氧化富集培养物主要由与蓝细菌门、浮霉菌门和变形菌门相关的操作分类单位主导。结论 我们的数据表明，PCL 可以作为富集 Mn 氧化微生物的潜在底物，使其能够在海洋条件下产生 MnOx。
Objective Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolactone (PCL), an aliphatic polyester, as an effective solid organic substrate for the enrichment of marine Mn-oxidizing microorganisms. Results We successfully obtained marine microbial enrichment with the capacity for dissolved Mn removal and MnOx production using PCL as a solid organic substrate. The removal of dissolved Mn by the Mn-oxidizing enrichment culture followed first-order kinetics with a rate constant of 0.014 h(-1). 16S rRNA gene amplicon sequencing analysis revealed that the Mn-oxidizing enrichment culture was highly dominated by operational taxonomic units related to the bacterial phyla Cyanobacteria, Planctomycetes, and Proteobacteria. Conclusions Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions.