Immune cells in the human choroid
Immune cells in the human choroid
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DOI：
10.1136/bjo.2007.129742
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发表时间：
2008-07-01
影响因子：
4.1
通讯作者：
Pulido, J. S.
中科院分区：
文献类型：
--
作者：
Ezzat, M-K;Hann, C. R.;Pulido, J. S.
目的：研究有无玻璃体形成的黄斑脉络膜中白细胞的特征，以及晚期老年性黄斑变性(AMD)伴纤维血管瘢痕形成(FVS)患者的白细胞特征。结果：4只无玻璃体、4只有玻璃体和2只FVS的黄斑组织中CD45阳性细胞分别占23.9(SD 6.2)%、27.5(7.2)%和19.3(11.3)%。CD8阳性细胞分别为3.8(0.7)、6.8(2.2)和6.3(2.1)；CD14阳性细胞分别为3.7(3.7)、3.6(1.6)和2.6(3.6)。作者发现CD83阳性细胞仅在两只FVS中有一只眼有更严重的瘢痕。结论：无论有无玻璃体形成，人类脉络膜中含有相似数量的CD4阳性细胞和单核细胞，但CD8阳性细胞在有玻璃体形成的黄斑脉络膜中更为丰富。黄斑脉络膜中造血细胞的存在为炎症细胞可能参与AMD的发病机制提供了进一步的证据。
Aim: To characterise the leucocytes in human macular choroid with and without drusen, and in eyes with advanced age-related macular degeneration (AMD) with fibrovascular scarring (FVS).Methods: Ten eyes from nine donors ( range 55-91 years of age) were obtained from an eye bank within 38 h post mortem. Fixed macular biopsies were sectioned, stained immunochemically and examined for the presence of leucocyte antigens CD45, CD4, CD8, CD14 and CD83.Results: Four eyes without drusen, four eyes with drusen and two eyes with FVS contained 23.9 ( SD 6.2)%, 27.5 (7.2)%, and 19.3 (11.3)% CD45-positive cells, respectively. The corresponding percentages for CD4-positive cells were 5.4 (4.3), 8.9 (3.0) and 7.5 (8.1); for CD8-positive cells, 3.8 (0.7), 6.8 (2.2) and 6.3 (2.1); and for CD14-positive cells, 3.7 ( 3.7), 3.6 (1.6) and 2.6 ( 3.6), respectively. The authors found CD83-positive cells solely in one of the two FVS eyes examined that had the more severe form of scarring.Conclusion: Human choroid contains similar amounts of CD4-positive cells and monocytes irrespective of the presence of drusen, but CD8-positive cells are more abundant in macular choroid with drusen. The presence of haematopoietic cells in the macular choroid provides further evidence for the possible participation of inflammatory cells in pathogenesis of AMD.