Kinetic Control of Nucleosome Displacement by ISWI/ACF Chromatin Remodelers
Kinetic Control of Nucleosome Displacement by ISWI/ACF Chromatin Remodelers
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DOI：
10.1103/physrevlett.109.118103
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发表时间：
2012-09-13
影响因子：
8.6
通讯作者：
Blossey, Ralf
中科院分区：
文献类型：
--
作者：
Florescu, Ana-Maria;Schiessel, Helmut;Blossey, Ralf
染色质结构由染色质重塑剂动态组织，染色质重塑剂是移动和去除核小体的马达蛋白复合物。最近提出的重塑行动的监管基础的动力学校对计划相结合的识别组蛋白尾状态和ATP依赖性松动的DNA周围的核小体。ISWI-家族的重塑剂的成员另外识别核小体之间的接头长度。在这里，我们表明，额外的校对步骤，涉及连接器长度本身就足以促进核小体的规则阵列的形成。与统计定位相比，双向电机的ATP依赖性重塑显示出增强定位。
Chromatin structure is dynamically organized by chromatin remodelers, motor protein complexes which move and remove nucleosomes. The regulation of remodeler action has recently been proposed to underlie a kinetic proofreading scheme which combines the recognition of histone-tail states and the ATP-dependent loosening of DNA around nucleosomes. Members of the ISWI-family of remodelers additionally recognize linker length between nucleosomes. Here, we show that the additional proofreading step involving linker length alone is sufficient to promote the formation of regular arrays of nucleosomes. ATP-dependent remodeling by bidirectional motors is shown to reinforce positioning as compared to statistical positioning.