Milk ceruloplasmin and its expression by mammary gland and liver in pigs
Milk ceruloplasmin and its expression by mammary gland and liver in pigs
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DOI：
10.1006/abbi.1999.1572
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发表时间：
2000-01-15
影响因子：
3.9
通讯作者：
Wickler, SJ
中科院分区：
文献类型：
--
作者：
Cerveza, PJ;Mehrbod, F;Wickler, SJ
血浆铜蓝蛋白和铜在牛奶和血浆中的浓度，牛奶铜蓝蛋白的性质，和哺乳期和妊娠期对这些参数的影响，以及铜蓝蛋白mRNA的表达乳腺，在猪进行了检查。正如之前在人类中所观察到的，出生后几天母猪乳汁中的铜蓝蛋白和铜浓度远高于1个月后，产后第3天和第33天的平均铜蓝蛋白浓度分别为26.5和6.6 mg/L（通过免疫测定），总铜浓度分别为1.67和0.34 mg/L。血浆铜蓝蛋白氧化酶活性值（用对苯二胺测定）分别为7.8和1.3 nmol/min/L。每日牛奶铜蓝蛋白产量从61到22毫克/天，每日铜产量从38到12毫克/天，相比之下，在哺乳期或妊娠期血清铜蓝蛋白浓度变化不大或没有变化，虽然总血浆铜在妊娠结束时很高。通过SDS-PAGE和免疫印迹法测定，牛奶铜蓝蛋白与血清铜蓝蛋白具有相同的表观大小，通过北方分析和各种外显子的RT-PCR无法区分肝脏和乳腺的铜蓝蛋白mRNA。在哺乳期开始时显著增加，然后在下一个月下降，同时乳汁铜蓝蛋白产量下降。结果表明，牛奶铜蓝蛋白，而在血浆中相同的蛋白质，是不是来自血浆，而是由乳腺产生。(C)北京大学出版社.
Concentrations of ceruloplasmin and copper in milk and blood plasma, the nature of milk ceruloplasmin, and the effects of lactation and gestation on these parameters, as well as the expression of ceruloplasmin mRNA by the mammary gland, were examined in pigs. As seen previously in humans, ceruloplasmin and copper concentrations in sow milk were much higher a few days after birth than 1 month later, averaging 26.5 and 6.6 mg ceruloplasmin/L (by immunoassay) and 1.67 and 0.34 mg total Cu/L, on days 3 and 33 postpartum, respectively. Values for ceruloplasmin oxidase activity (measured with p-phenylene diamine) were 7.8 and 1.3 nmol/min/L, respectively. Daily milk ceruloplasmin production went from 61 to 22 mg/day and daily copper output from 38 to 12 mg/day, In contrast, there was little or no variation in serum ceruloplasmin concentration during lactation or gestation, although total plasma copper was high at the end of gestation. Milk ceruloplasmin was of the same apparent size as serum ceruloplasmin, as determined by SDS-PAGE and immunoblotting, and ceruloplasmin mRNAs of liver and mammary gland were indistinguishable by Northern analysis and RT-PCR of the various exons, Expression of total RNA and ceruloplasmin mRNA, as detected in biopsies of mammary gland, increased markedly upon onset of lactation and then declined during the next month in conjunction with a drop in milk ceruloplasmin production. The results indicate that milk ceruloplasmin, while being the same protein as in plasma, is not derived from the plasma but is produced by the mammary gland. (C) 2000 Academic Press.