Orally Administered Glucosylceramide Improves the Skin Barrier Function by Upregulating Genes Associated with the Tight Junction and Cornified Envelope Formation
Orally Administered Glucosylceramide Improves the Skin Barrier Function by Upregulating Genes Associated with the Tight Junction and Cornified Envelope Formation
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DOI：
10.1271/bbb.110215
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发表时间：
2011-08-01
影响因子：
1.6
通讯作者：
Uchiyama, Taro
中科院分区：
文献类型：
--
作者：
Ideta, Ritsuro;Sakuta, Tomohiro;Uchiyama, Taro
膳食葡萄糖神经酰胺可改善皮肤屏障功能。我们使用微阵列系统来分析经 SDS 处理的无毛小鼠背部皮肤中的 mRNA 表达，以阐明所涉及的分子机制。 SDS处理增加了小鼠皮肤的经皮水分流失，通过预先口服葡萄糖神经酰胺显着减少了这种增加。微阵列评估的 mRNA 表达比显示，与喂食葡萄糖神经酰胺/SDS 处理的小鼠皮肤中的所有基因相比，与角质化包膜和紧密连接形成相关的基因的表达在统计学上显着增加。然后我们检查了葡萄糖神经酰胺代谢物对培养的角质形成细胞紧密连接形成的贡献。培养的角质形成细胞的 SDS 处理显着降低了经表皮电阻，这种降低在鞘氨醇或植物鞘氨醇（葡萄糖神经酰胺的主要代谢物）存在下得到显着改善。这些结果表明，口服葡萄糖神经酰胺通过上调与角质化包膜和紧密连接形成相关的基因来改善皮肤屏障功能。
Dietary glucosylceramide improves the skin barrier function. We used a microarray system to analyze the mRNA expression in SDS-treated dorsal skin of the hairless mouse to elucidate the molecular mechanisms involved. The transepidermal water loss of mouse skin was increased by the SDS treatment, this increase being significantly reduced by a prior oral administration of glucosylceramides. The microarray-evaluated mRNA expression ratio showed a statistically significant increase in the expression of genes related to the cornified envelope and tight junction formation when compared with all genes in the glucosylceramide-fed/SDS-treated mouse skin. We then examined the contribution of glucosylceramide metabolites to the tight junction formation of cultured keratinocytes. The SDS treatment of cultured keratinocytes significantly decreased the transepidermal electrical resistance, this decrease being significantly ameliorated in the presence of sphingosine or phytosphingosine, the major metabolites of glucosylceramide. These results suggest that an oral administration of glucosylceramide improved the skin barrier function by up-regulating genes associated with both the cornified envelope and tight junction formation.