Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures
Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures
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DOI：
10.1016/j.actbio.2008.08.015
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发表时间：
2009-02-01
影响因子：
9.7
通讯作者：
Fernandes, M. H.
中科院分区：
文献类型：
--
作者：
Amaral, M.;Gomes, P. S.;Fernandes, M. H.
研究了纳米金刚石(NCD)涂层在Si(3)N(4)陶瓷表面的细胞毒性。这种材料被设想用于生物医学牙科应用，如毛刺和手术器械。使用两种成纤维细胞培养系统来研究NCD涂层样品的细胞毒性：L929细胞(小鼠永久细胞系)和人牙龈成纤维细胞。通过细胞黏附、细胞存活/增殖(线粒体功能，四甲基偶氮唑盐比色法)和细胞生长模式来评估细胞行为。成纤维细胞在标准聚苯乙烯培养板上的行为被用作对照，因为以前已经证明Si3N4底物具有生物相容性。NCD涂层为细胞的附着、铺展和增殖提供了合适的表面。人牙龈细胞在融合培养上表现出均匀的细胞质铺展，扁平拉长的形态和典型的平行排列。与之相比，L929细胞的胞浆扩张率较低，呈异质性扩散，但增殖率较高。两种细胞在几天后，NCD涂层都被连续的细胞层完全覆盖。与标准聚苯乙烯培养板相比，L929和人成纤维细胞培养均未观察到有害或细胞毒性反应，细胞增殖略有增强。此外，种植的NCD膜允许复制两种测试的细胞培养系统的典型特征，进一步表明这种涂层没有细胞毒性。(C)2008 Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
The cytotoxicity profile of nanocrystalline diamond (NCD) coatings on a Si(3)N(4) ceramic was investigated. This material is envisaged to have biomedical dental applications such as burrs and surgical instruments. Two fibroblast cell culture systems were used to address the cytotoxicity of NCD-coated samples: L929 cells (a mouse permanent cell line) and human gingival fibroblasts. Cell behavior was evaluated in terms of cell adhesion, cell viability/proliferation (mitochondrial function, MTT assay) and the pattern of cell growth. Fibroblast cell behavior on standard polystyrene culture plates was used as control, as Si3N4 substrates have previously been shown to be biocompatible. NCD coatings provided a suitable surface for cell attachment, spreading and proliferation. Human gingival cells showed a homogeneous cytoplasm spreading, a flattened elongated morphology and a typical parallel alignment on confluent cultures. In comparison, L929 cells denoted a lower cytoplasm expansion, a heterogeneous spreading but a higher proliferation rate. For both cells, after few days, the NCD coating was completely covered with continuous cell layers. As compared to standard polystyrene culture plates, no deleterious or cytotoxic responses were observed with L929 and human fibroblast cell cultures, and in both a slight enhancement in cell proliferation was observed. In addition, the seeded NCD film allowed reproduction of the typical features of the two cell culture systems tested, further suggesting the lack of cytotoxicity of this coating. (c) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.