Late phase bronchial obstruction following nonimmunologic mast cell degranulation.
Late phase bronchial obstruction following nonimmunologic mast cell degranulation.
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非免疫性肥大细胞脱颗粒后的晚期支气管阻塞。
DOI：
10.1152/jappl.1984.57.4.1182
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
W. Abraham
中科院分区：
文献类型：
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作者：
E. Russi;A. Perruchoud;L. Yerger;J. Stevenson;J. Tabak;B. Marchette;W. Abraham
吸入抗原后气道肥大细胞的免疫脱颗粒会导致过敏性绵羊的早期和晚期气道阻塞。在本研究中，我们确定吸入化合物 48/80 引起的气道肥大细胞的非免疫脱颗粒是否具有类似的效果。在五只羊中，在吸入 48/80 气雾剂之前和之后的预定时间测定肺流阻 (RL)、胸腔气体体积 (Vtg) 和动脉氧张力 (Pao2)。攻击后，平均比肺阻力 (sRL = RL X Vtg) 立即增加 259%，平均 Pao2 下降 29%。 3 小时后所有值均恢复正常。到攻击后 5 小时，sRL 再次显着增加； sRL 的第二次增加（高于基线 92%）在 7 小时时达到最大，同时 Pao2 下降 17%。在这些相同的绵羊中，吸入猪蛔虫抗原会产生类似的早期 sRL 变化，但晚期反应的发生有所延迟且更为明显。在第二组绵羊（n = 5）中，用肥大细胞稳定剂色甘酸钠进行预处理可防止化合物 48/80 的早期和晚期反应。用组胺 H1 拮抗剂扑尔敏进行预处理对这两种反应均没有显着影响，而用过敏反应慢反应物质 (SRS-A) 拮抗剂 FPL 55712 进行预处理，可轻微但不显着减弱早期反应，并完全阻止晚期反应。我们的结论是，与免疫刺激一样，非免疫性肥大细胞脱颗粒会在过敏性绵羊中产生早期和晚期支气管阻塞。这些反应是依赖于中介者的；虽然组胺和 SRS-A 有助于早期反应，但 SRS-A 的早期出现是晚期反应的重要先决条件。
Immunologic degranulation of airway mast cells after antigen inhalation produces early and late airway obstructions in allergic sheep. In this study we determined whether nonimmunologic degranulation of airway mast cells by inhalation of compound 48/80 had similar effects. In five sheep, pulmonary flow resistance (RL), thoracic gas volume (Vtg), and arterial O2 tension (Pao2) were determined prior to and at predetermined times after inhalation of 48/80 aerosol. Immediately after challenge mean specific lung resistance (sRL = RL X Vtg) increased by 259% and mean Pao2 decreased by 29%. All values returned to normal by 3 h. By 5-h postchallenge sRL again increased significantly; this second increase in sRL (92% above base line) was maximal at 7 h and was accompanied by a 17% drop in Pao2. In these same sheep inhalation of Ascaris suum antigen produced comparable early changes in sRL, but the onset of the late response was somewhat delayed and more pronounced. In a second group of sheep (n = 5), pretreatment with the mast cell stabilizer cromolyn sodium prevented both early and late responses by compound 48/80. Pretreatment with the histamine H1-antagonist chlorpheniramine had no significant effect on either response, whereas pretreatment with FPL 55712, an antagonist of slow-reacting substance of anaphylaxis (SRS-A), slightly but not significantly attenuated the early response and completely prevented the late response. We conclude that, like immunologic stimuli, nonimmunologic mast cell degranulation produces early and late bronchial obstructions in allergic sheep; that these responses are mediator dependent; and that while histamine and SRS-A contribute to the early response, it is the early appearance of SRS-A which is an important prerequisite for the late response.