Myofibroblast induction with transforming growth factor-β1 and -β3 in cutaneous fetal excisional wounds
Myofibroblast induction with transforming growth factor-β1 and -β3 in cutaneous fetal excisional wounds
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DOI：
10.1016/s0022-3468(00)90007-1
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发表时间：
2000-02-01
影响因子：
2.4
通讯作者：
Haynes, JH
中科院分区：
文献类型：
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作者：
Lanning, DA;Diegelmann, RF;Haynes, JH
背景/目的：在一个无收缩的胎兔模型中，作者最近发现了用转化生长因子-β制成的缓释纤维素植入物诱导创面切除收缩。本研究的目的是验证该模型中创面收缩与周围真皮成肌细胞的诱导有关的假说，该假说表现为α-平滑肌动蛋白的存在。方法：将含有1.0mgTGF-β(1)(n=6)、1.0mgTGF-β(3)(n=9)、10mUg(3)(n=6)或其载体蛋白牛血清白蛋白(BSA；n=9)的纤维素片制成缓释5天以上。在妊娠24天(足月，31天)，将每个圆盘植入胚胎新西兰白兔背部的皮下口袋中。然后在植入的纤维素盘旁边制作一个全厚、3 mm的切除伤口(7.4 mm(2))。所有胎儿均于3天后取材。结果：术后创面分别加入1.0mgTGF-β(1)(5.6+/-2.0 mm(2))、1.0mgTGF-β(3)(6.9+/-1.0 mm(2))，10mgTGF-β(3)(2.7+/-1.0 mm(2))明显小于BSA对照组(12.8+/-1.1 mm(2))；P
Background/Purpose: In a noncontractile fetal rabbit model, the authors recently have shown the induction of excisional wound contraction with sustained-release cellulose implants formulated with transforming growth factor (TGF)-beta. The purpose of this study was to test the hypothesis that the excisional wound contraction in this model is associated with the induction of myofibroblasts in the surrounding dermis, demonstrated by the presence of alpha-smooth muscle actin.Methods: Cellulose discs were formulated with either 1.0 mu g of TGF-beta(1) (n = 6); 1.0 mu g of TGF-beta(3) (n = 9); 10 mu g of TGF-beta(3) (n = 6); or their carrier protein, bovine serum albumin (BSA; n = 9), for sustained-release over 5 days. Each disc was implanted into a subcutaneous pocket on the back of a fetal New Zealand White rabbit in utero on day 24 of gestation (term, 31 days). A full-thickness, 3-mm excisional wound (7.4 mm(2)) was then made next to the implanted cellulose disc. All fetuses were harvested at 3 days. The amount of alpha-smooth muscle (SM) actin in the dermis around the implants and wounds was determined using immunohistochemical techniques.Results: Excisional wounds exposed to 1.0 mu g of TGF-beta(1) (5.6 +/- 2.0 mm(2)), 1.0 mu g of TGF-beta(3) (6.9 +/- 1.0 mm(2)), and 10 mu g of TGF-beta(3) (2.7 +/- 1.0 mm(2)) were significantly smaller when compared with the BSA control group (12.8 +/- 1.1 mm(2); P