Highly stable and degradable multifunctional microgel for self-regulated insulin delivery under physiological conditions
Highly stable and degradable multifunctional microgel for self-regulated insulin delivery under physiological conditions
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高度稳定且可降解的多功能微凝胶，用于生理条件下自我调节的胰岛素输送
DOI：
10.1039/c3nr00835e
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发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Liu, Mingzhu
中科院分区：
文献类型：
--
作者：
Zhang, Xinjie;Lu, Shaoyu;Liu, Mingzhu
通过合理设计，在胶体化学方法中应用多功能微凝胶，可以同时实现对葡萄糖、pH和温度的响应、高载药量、自我调节的药物递送和体内降解。以N-异丙基丙烯酰胺（NIPAAm）、甲基丙烯酸（2-二甲氨基）乙酯（DMAEMA）和3-丙烯酰胺基苯硼酸（AAPBA）为原料，采用沉淀乳液法制备了多功能微凝胶，并用可还原降解的N，N '-双（芳酰基）胱胺（BAC）进行交联。这种具有窄尺寸分布（类似于250 nm）的新型微凝胶适用于糖尿病，因为它可以适应临床相关范围（0-20 mM）内不同葡萄糖浓度的周围介质，控制预载胰岛素的释放，并且在生理条件（pH 7.4，0.15 M NaCl，37 ℃）下高度稳定。当合成的多功能微凝胶调节药物递送时，它们随着时间的推移而逐渐降解，因此显示出增强的生物相容性。这为糖尿病治疗提供了一种新的概念验证，它利用了多功能微物体的每个构建块的特性。这些高度稳定和多功能的多功能微凝胶有可能用于自我调节治疗和监测对治疗的反应，甚至作为纳米生物传感器进行同步诊断。
The response to glucose, pH and temperature, high drug loading capacity, self-regulated drug delivery and degradation in vivo are simultaneously probable by applying a multifunctional microgel under a rational design in a colloid chemistry method. Such multifunctional microgels are fabricated with N-isopropylacrylamide (NIPAAm), (2-dimethylamino)ethyl methacrylate (DMAEMA) and 3-acrylamidephenylboronic acid (AAPBA) through a precipitation emulsion method and cross-linked by reductive degradable N,N'-bis(arcyloyl)cystamine (BAC). This novel kind of microgel with a narrow size distribution (similar to 250 nm) is suitable for diabetes because it can adapt to the surrounding medium of different glucose concentrations over a clinically relevant range (0-20 mM), control the release of preloaded insulin and is highly stable under physiological conditions (pH 7.4, 0.15 M NaCl, 37 degrees C). When synthesized multifunctional microgels regulate drug delivery, they gradually degrade as time passes and, as a result, show enhanced biocompatibility. This exhibits a new proof-of-concept for diabetes treatment that takes advantage of the properties of each building block from a multifunctional micro-object. These highly stable and versatile multifunctional microgels have the potential to be used for self-regulated therapy and monitoring of the response to treatment, or even simultaneous diagnosis as nanobiosensors.