Three populations of migrating amphibian embryonic cells utilize different guidance cues.
Three populations of migrating amphibian embryonic cells utilize different guidance cues.
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三个迁移两栖动物胚胎细胞群体利用不同的引导信号。
DOI：
10.1006/dbio.1993.1272
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发表时间：
1993
影响因子：
2.7
通讯作者：
Steinberg,MS
中科院分区：
文献类型：
--
作者：
Thibaudeau,G;Drawbridge,J;Dollarhide,AW;Haque,T;Steinberg,MS
以前的研究旨在确定分子（S）管理的两栖动物原肾管（PND）的定向迁移揭示了糖基磷脂酰肌醇（GPI）连接的细胞表面分子，可能是胞外碱性磷酸酶（AP）的要求。移植到侧腹的颅神经嵴细胞（CNC）与PND沿沿着相同的途径迁移，表明PND和CNC引导系统可能具有共同的分子基础。PND和CNC迁移途径均显示AP。然而，目前的实验表明，这些途径上的GPI连接的分子对于两种细胞类型的迁移都不是必需的。由于PND细胞本身显著表达AP，而CNC细胞不表达，我们询问PND迁移所需的GPI连接分子是否存在于PND细胞本身。用磷脂酰肌醇特异性磷脂酶C（一种去除GPI连接蛋白的酶）处理PND细胞，可以阻止它们的迁移。移植实验表明，虽然CNC细胞能够遵循PND迁移途径，但情况并非如此。将移植实验扩展到包括躯干神经嵴（TNC）细胞表明，尽管CNC细胞可以在侧腹遵循PND指导信息，但PND、CNC和TNC细胞通常都利用不同的分子线索来指导它们的原位迁移。
Previous investigations designed to identify the molecule(s) governing the directed migration of the amphibian pronephric duct (PND) revealed a requirement for a glycosyl phosphatidylinositol (GPI)-linked cell surface molecule, possibly the ectoenzyme alkaline phosphatase (AP). Cranial neural crest cells (CNC) grafted to the flank migrate along the same pathways as the PND, suggesting that PND and CNC guidance systems might have a common molecular basis. Both PND and CNC migration pathways display AP. The present experiments demonstrate, however, that GPI-linked molecules on these pathways are not required for migration of either cell type. Because PND cells themselves express AP prominently but CNC cells do not, we asked whether the GPI-linked molecule required for PND migration resides on the PND cells themselves. Treatment of PND cells with phosphatidylinositol-specific phospholipase C, an enzyme that removes GPI-linked proteins, prevents their migration. Transplantation experiments show that although CNC cells are capable of following PND migration pathways, the converse is not the case. Extension of the transplantation experiments to include trunk neural crest (TNC) cells indicates that although CNC cells can follow PND guidance information on the flank, PND, CNC, and TNC cells all normally utilize different molecular cues to guide their migrationsin situ.