CATABOLITE REPRESSION OF ALPHA-AMYLASE GENE-EXPRESSION IN BACILLUS-SUBTILIS INVOLVES A TRANS-ACTING GENE-PRODUCT HOMOLOGOUS TO THE ESCHERICHIA-COLI LACL AND GALR REPRESSORS
CATABOLITE REPRESSION OF ALPHA-AMYLASE GENE-EXPRESSION IN BACILLUS-SUBTILIS INVOLVES A TRANS-ACTING GENE-PRODUCT HOMOLOGOUS TO THE ESCHERICHIA-COLI LACL AND GALR REPRESSORS
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DOI：
10.1111/j.1365-2958.1991.tb00728.x
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发表时间：
1991-03-01
影响因子：
3.6
通讯作者：
CHAMBLISS, GH
中科院分区：
文献类型：
--
作者：
HENKIN, TM;GRUNDY, FJ;CHAMBLISS, GH
枯草芽孢杆菌α-淀粉酶基因的表达被控制在转录水平，并响应细胞的生长状态以及快速代谢碳源的可用性。先前已经证明，葡萄糖介导的抑制涉及AMIE基因转录起始点附近的一个位置。在这项研究中，我们发现了一个转座子插入突变，该突变导致AMYE基因表达的葡萄糖抑制缺失。该突变位于枯草芽孢杆菌染色体图谱的263度附近，对该突变所影响的基因进行了分离和DNA测序。该基因命名为CCPA，与Lac和Gal抑制因子家族的抑制因子基因具有显著的同源性。CCPA基因被发现与ALSA等位基因，ALSA以前被认为是乙酸乙酯生物合成的调节因子，也可能参与其他系统的分解代谢调节。
Expression of the alpha-amylase gene of Bacillus subtilis is controlled at the transcriptional level, and responds to the growth state of the cell as well as the availability of rapidly metabolizable carbon sources. Glucose-mediated repression has previously been shown to involve a site near the transcriptional start-point of the amyE gene. In this study, a transposon insertion mutation was characterized which resulted in loss of glucose repression of amyE gene expression. The gene affected by this mutation, which was localized near 263-degrees on the B. subtilis chromosomal map, was isolated and its DNA sequence was determined. This gene, designated ccpA, exhibited striking homology to repressor genes of the lac and gal repressor family. The ccpA gene was found to be allelic to alsA, previously identified as a regulator of acetoin bio-synthesis, and may be involved in catabolite regulation of other systems as well.