PHYLOGENETIC ANALYSIS OF SORGHUM AND RELATED TAXA USING INTERNAL TRANSCRIBED SPACERS OF NUCLEAR RIBOSOMAL DNA
PHYLOGENETIC ANALYSIS OF SORGHUM AND RELATED TAXA USING INTERNAL TRANSCRIBED SPACERS OF NUCLEAR RIBOSOMAL DNA
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DOI：
10.1007/bf00226978
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发表时间：
1994-09-01
影响因子：
5.4
通讯作者：
HULBERT, SH
中科院分区：
文献类型：
--
作者：
SUN, Y;SKINNER, DZ;HULBERT, SH
利用核糖体DNA（rDNA）内转录间隔区（ITS）序列分析，研究了高粱属及其近缘属的系统发育关系。从15份高粱属材料中提取DNA，其中包括来自Chaetosaurum和Heterosaurum组的各一份材料，来自副高粱的四份材料，来自杆高粱的两份材料，以及来自高粱组的三个种的七份代表材料（S. propinquum和S. halepense和S. bicolor）。玉米（Zen mays）系H95和来自高粱水葱的加入物也包括在研究中。可变核苷酸被用来构建一个严格的共识系统发育树。分析表明，S. propinquum，S. halepense和S.双色亚种arundinaceum race aethiopicum可能是栽培高粱的最接近的野生近缘种，Sordinaceum nitidum可能是栽培高粱的最接近的2n=10近缘种。双色; Chaetosaurum组和Heterosaurum组似乎彼此密切相关，并且与Soraum组的关系比与Parasoraum组的关系更密切;并且Parasoraum组不是单系的。这些结果还表明，高粱属是一个非常古老和多样的组。
The phylogenetic relationships of the genus Sorghum and related genera were studied by sequencing the nuclear ribosomal DNA (rDNA) internal transcribed spacer region (ITS). DNA was extracted from 15 Sorghum accessions, including one accession from each of the sections Chaetosorghum and Heterosorghum, four accessions from Parasorghum, two accessions from Stiposorghum, and seven representatives from three species of the section Sorghum (one accession from each of S. propinquum and S. halepense, and five races of S. bicolor). The maize (Zen mays) line, H95, and an accession from Cleistachne sorghoides were also included in the study. Variable nucleotides were used to construct a strict consensus phylogenetic tree. The analyses indicate that S. propinquum, S. halepense and S. bicolor subsp. arundinaceum race aethiopicum may be the closest wild relatives of cultivated sorghum; Sorghum nitidum may be the closest 2n=10 relative to S. bicolor; the sections Chaetosorghum and Heterosorghum appear closely related to each other and more closely related to the section Sorghum than Parasorghum; and the section Parasorghum is not monophyletic. The results also indicate that the genus Sorghum is a very ancient and diverse group.