Spatiotemporal and species variations in prokaryotic communities associated with sediments from surface-flow constructed wetlands for treating swine wastewater.
Spatiotemporal and species variations in prokaryotic communities associated with sediments from surface-flow constructed wetlands for treating swine wastewater.
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DOI：
10.1016/j.chemosphere.2017.06.132
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发表时间：
2017-10
期刊：
影响因子：
8.8
通讯作者：
Fen Jia;Cui Lai;Liang Chen;G. Zeng;Danlian Huang;Feng Liu;Xi Li;Pei Luo;Jinshui Wu;L. Qin;Chen Zhang;Min Cheng;Piao Xu
中科院分区：
文献类型：
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作者：
Fen Jia;Cui Lai;Liang Chen;G. Zeng;Danlian Huang;Feng Liu;Xi Li;Pei Luo;Jinshui Wu;L. Qin;Chen Zhang;Min Cheng;Piao Xu
微生物是污水处理人工湿地去除污染物的主要机制。然而，不同的生物过程和原核生物群落的变化仍然知之甚少。在这项研究中，我们应用高通量测序技术研究了中试规模的地表流人工湿地（SFCWs）处理不同强度猪废水（SW）的沉积物相关的原核生物群落。结果表明，SFCWs原核生物群落高度多样化，变形菌门（16.44 ~ 44.44%）、酸菌门（3.25 ~ 24.40%）和绿菌门（5.77 ~ 14.43%）为主要门，硝化螺门（4.14 ~ 12.02%）为最优势属。沉积物中原核生物群落随地点和季节变化较大，微环境条件发生显著变化。主坐标分析表明，SW强度对SFCWs沉积物群落结构有显著影响，典型对应分析表明，冬季原核生物群落的变化与NO3−-N和TN密切相关；夏季NH4+单键dn、NO3−-N、NO2−-N、TN、TP、SOM和ph。总之，高通量测序的使用极大地增强了我们对SFCWs中不同官能团原核生物群落的认识。
Microorganisms are the main mechanisms of pollutants removals in constructed wetlands (CWs) used for wastewater treatment. However, the different biological processes and variations of prokaryotic community in CWs remain poorly understood. In this study, we applied a high-throughput sequencing technique to investigate the prokaryotic communities associated with sediments from pilot-scale surface-flow constructed wetlands (SFCWs) treating swine wastewater (SW) of varying strengths. Our results revealed that highly diverse prokaryotic communities were present in the SFCWs, withProteobacteria(16.44–44.44%),Acidobacteria(3.25–24.40%), andChloroflexi(5.77–14.43%) being the major phyla, andNitrospira(4.14–12.02%), the most dominant genus. The prokaryotic communities in the sediments varied greatly with location and season, which markedly altered the microenvironmental conditions. Principal co-ordinates analysis indicated that SW strength significantly influenced the community structure in sediments of the SFCWs, and canonical correspondence analysis illustrated that the shifts in prokaryotic communities were strongly related to NO3−–N and TN in winter; and in summer with NH4+single bondN, NO3−–N, NO2−–N, TN, TP, SOM, and pH. In conclusion, the use of high-throughput sequencing greatly enhanced our understanding of prokaryotic communities with different functional groups in SFCWs.