Genome-Wide Analysis of Nutrient Signaling Pathways Conserved in Arbuscular Mycorrhizal Fungi.
Genome-Wide Analysis of Nutrient Signaling Pathways Conserved in Arbuscular Mycorrhizal Fungi.
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丛枝菌根真菌中保守的营养信号通路的全基因组分析
DOI：
10.3390/microorganisms9081557
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发表时间：
2021-07-22
期刊：
影响因子：
4.5
通讯作者：
Xie X
中科院分区：
文献类型：
--
作者：
Zhou X;Li J;Tang N;Xie H;Fan X;Chen H;Tang M;Xie X
丛枝菌根（AM）真菌与大多数陆生维管植物形成互惠共生关系。为了实现与宿主的有效营养交换，AM真菌感知外部和内部营养，并整合不同的等级调节以优化菌根化过程中的营养获取和稳态。然而，AM真菌中协调营养感测和信号传导的潜在分子网络仍然难以捉摸。通过同源性检索发现，AM真菌中至少有72个基因组分在cAMP依赖性蛋白激酶A（cAMP-PKA）、蔗糖非发酵蛋白激酶1（SNF 1）、雷帕霉素靶激酶（TOR）和磷酸盐（PHO）信号级联等4条营养传感和信号通路中高度保守。基于已知的知识在模式酵母和丝状真菌，我们概述了可能的基因网络功能的AM真菌。这些途径可能调控下游基因的表达，这些基因参与营养物质的运输、脂质代谢、海藻糖酶活性、抗逆和自噬。部分核心基因的RNA-seq分析和qRT-PCR结果进一步表明，这些途径可能在AM真菌孢子萌发、附着胞形成、丛枝寿命和产孢过程中发挥重要作用。我们希望这些候选基因在AM真菌和AM共生中参与这些营养传感和信号转导途径的作用能启发进一步的研究。
Arbuscular mycorrhizal (AM) fungi form a mutualistic symbiosis with a majority of terrestrial vascular plants. To achieve an efficient nutrient trade with their hosts, AM fungi sense external and internal nutrients, and integrate different hierarchic regulations to optimize nutrient acquisition and homeostasis during mycorrhization. However, the underlying molecular networks in AM fungi orchestrating the nutrient sensing and signaling remain elusive. Based on homology search, we here found that at least 72 gene components involved in four nutrient sensing and signaling pathways, including cAMP-dependent protein kinase A (cAMP-PKA), sucrose non-fermenting 1 (SNF1) protein kinase, target of rapamycin kinase (TOR) and phosphate (PHO) signaling cascades, are well conserved in AM fungi. Based on the knowledge known in model yeast and filamentous fungi, we outlined the possible gene networks functioning in AM fungi. These pathways may regulate the expression of downstream genes involved in nutrient transport, lipid metabolism, trehalase activity, stress resistance and autophagy. The RNA-seq analysis and qRT-PCR results of some core genes further indicate that these pathways may play important roles in spore germination, appressorium formation, arbuscule longevity and sporulation of AM fungi. We hope to inspire further studies on the roles of these candidate genes involved in these nutrient sensing and signaling pathways in AM fungi and AM symbiosis.
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