The bioactivity of teleost IL-6: IL-6 protein in orange-spotted grouper (Epinephelus coioides) induces Th2 cell differentiation pathway and antibody production
The bioactivity of teleost IL-6: IL-6 protein in orange-spotted grouper (Epinephelus coioides) induces Th2 cell differentiation pathway and antibody production
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DOI：
10.1016/j.dci.2012.06.013
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发表时间：
2012-10-01
影响因子：
2.9
通讯作者：
Lin, John Han-You
中科院分区：
文献类型：
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作者：
Chen, Hsin-Hung;Lin, Han-Tso;Lin, John Han-You
白细胞介素6（IL-6）是由T细胞和巨噬细胞分泌的蛋白质，在免疫应答中起重要作用。IL-6调节T细胞的增殖和分化，并刺激B细胞中免疫球蛋白的产生。本研究获得了橙子石斑鱼IL-6（gil-6）cDNA序列。推导的IL-6（gIL-6）蛋白由223个氨基酸组成，与哺乳动物IL-6的同源性约为30%，与其它硬骨鱼IL-6的同源性在47%~ 69%之间。该蛋白质包含信号肽、IL-6家族标记和在其它硬骨鱼类的IL-6序列中发现的保守氨基酸残基。为了解gIL-6的生物活性及其对体液免疫应答的影响，将原核生物合成的重组gIL-6（rgIL-6）注射到橙子石斑鱼体内，观察免疫相关基因在不同时间各器官中的表达。结果表明，IL-6刺激后，免疫器官中Th 1特异性转录因子t-bet表达下调，Th 2特异性转录因子gata 3和c-maf表达上调。此外，在rgIL-6刺激的鱼中检测到较高水平的igm mRNA和翻译蛋白。这些结果表明，IL-6在石斑鱼调节幼稚T辅助细胞分化为Th 2细胞和增强抗体的产生。(C)2012爱思唯尔有限公司保留所有权利。
Interleukin 6 (IL-6) is a protein secreted by T cells and macrophages and plays an important role in immune response. IL-6 regulates the proliferation and differentiation of T cells, and elicits immunoglobulin production in B cells. In this study, the cDNA il-6 (gil-6) sequence of the orange spotted grouper (Epinephelus coioides) was obtained. The deduced IL-6 (gIL-6) protein comprised 223 amino acids, the sequence shared approximately 30% similarity with mammalian IL-6, and between 47% and 69% similarity with other available teleost IL-6. The protein comprises the signal peptide, the IL-6 family signature, and conserved amino acid residues found in IL-6 sequences of other teleost. In order to understand the bioactivity and influence of gIL-6 on humoral immune response, recombinant gIL-6 (rgIL-6) synthesized by prokaryotes was injected into orange spotted groupers, and the immune-related gene expression at various times in various organs was observed. Our results revealed that the Th1 specific transcription factor t-bet was down-regulated and Th2 specific transcription factors gata3, and c-maf were up-regulated in immune organs, following IL-6 stimulation. Additionally, higher levels of igm mRNA and translated protein were detected in rgIL-6 stimulated fish. These results indicate that IL-6 in groupers regulates the differentiation of naive T helper cells into Th2 cells and elicits the production of antibodies. (C) 2012 Elsevier Ltd. All rights reserved.