Bacteriophage WO and virus-like particles in Wolbachia, an endosymbiont of arthropods
Bacteriophage WO and virus-like particles in Wolbachia, an endosymbiont of arthropods
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DOI：
10.1006/bbrc.2001.4906
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发表时间：
2001-05-25
影响因子：
3.1
通讯作者：
Ishikawa, H
中科院分区：
文献类型：
--
作者：
Masui, S;Kuroiwa, H;Ishikawa, H
沃尔巴克氏体是一种主要存在于节肢动物体内的细胞内共生体，通过未知的机制引起宿主的各种性别改变。在这里，我们报告的结果，强烈表明，沃尔巴克氏体有病毒样颗粒的噬菌体WO，这是以前确定为一个前噬菌体样元件在沃尔巴克氏体基因组。首次用抗Wolbachia外表面蛋白Wsp的抗体，用荧光显微镜和免疫电镜技术检测了昆虫体内Wolbachia（wTai株）的感染。电镜下观察到沃尔巴克氏体中的病毒样颗粒。昆虫卵巢的0.22 μ m滤液含有DAPI阳性颗粒，PCR分析表明噬菌体WO DNA通过过滤器，而沃尔巴克氏体DNA被消除，这表明DAPI阳性颗粒是噬菌体WO，（C）2001 Academic Press。
Wolbachia are intracellular symbionts mainly found in arthropods, causing various sexual alterations on their hosts by unknown mechanisms. Here we report the results that strongly suggest that Wolbachia have virus-like particles of phage WO, which was previously identified as a prophage-like element in the Wolbachia genome. Wolbachia (strain wTai) infection in an insect was detected with the antibody against Wsp, an outer surface protein of Wolbachia, by fluorescence microscopy and immunoelectron-microscopy for the first time. Virus-like particles in Wolbachia were observed by electron-microscopy. The 0.22-mum filtrate of insect ovary contained DAPI-positive particles, and PCR analysis demonstrated that a phage WO DNA passed through the filter while Wolbachia DNA were eliminated, suggesting that the DAPI-positive particles were phage WO, (C) 2001 Academic Press.