Ruthenibacterium lactatiformans gen. nov., sp nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces
Ruthenibacterium lactatiformans gen. nov., sp nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces
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DOI：
10.1099/ijsem.0.001143
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发表时间：
2016-08-01
影响因子：
2.8
通讯作者：
Efimov, Boris A.
中科院分区：
文献类型：
--
作者：
Shkoporov, Andrei N.;Chaplin, Andrei V.;Efimov, Boris A.
从健康人粪便中分离到两株革兰氏染色阴性、杆状、厌氧、不形成芽胞、不能运动的新菌株。这些菌株命名为585-1(T)和668，具有中温发酵代谢、D-葡萄糖发酵的最终产物D-乳酸、琥珀酸和醋酸的产生，C-18：1欧米伽9，C-18：1欧米伽9醛，C-16：0和C-16：1欧米伽7C脂肪酸的存在，多聚糖多糖肽部分中存在甘氨酸、谷氨酸、赖氨酸、丙氨酸和天冬氨酸，缺乏呼吸醌。全基因组测序结果显示，DNA G+C含量为56.4-56.6摩尔%。两株菌的16SRRNA基因全序列与戊型厌氧菌Fae(T)、甲酸根霉ATCC 27749(T)和普氏链球菌ATCC 27768(T)的同源性分别为91.7/91.6%、91.3/91.2%和88.9/88.8%。根据化学分类学和基因组学特征，这些菌株是反刍球菌科一个新属的新种，命名为乳酸鲁氏杆菌。Nov.，sp.11月是提议的。乳酸红杆菌模式菌株为585-1(T)(=DSM 100348(T)=VKM B-2901(T))。
Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1(T) and 668, were characterized by mesophilic fermentative metabolism, production of D-lactic acid, succinic acid and acetic acid as end products of D-glucose fermentation, prevalence of C-18 : 1 omega 9, C-18 : 1 omega 9 aldehyde, C-16 : 0 and C-16 : 1 omega 7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4-56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6% similarity with Anaerofilum pentosovorans Fae(T), 91.3/91.2% with Gemmiger formicilis ATCC 27749(T) and 88.9/88.8% with Faecalibacterium prausnitzii ATCC 27768(T). On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae, for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1(T) (=DSM 100348(T) =VKM B-2901(T)).