Formation of flagella lacking outer rings by flaM, flaU, and flaY mutants of Escherichia coli
Formation of flagella lacking outer rings by flaM, flaU, and flaY mutants of Escherichia coli
复制标题
大肠杆菌 flaM、flaU 和 flaY 突变体形成缺乏外环的鞭毛
作者：
K. Ohnishi;M. Homma;K. Kutsukake;T. Iino
在大肠杆菌的鞭毛突变体中，flaM或flaU突变体形成缺乏外P和L环的基体，而flaY突变体主要形成缺乏L环的基体。在这些突变体中，钩和丝偶尔会组装到这些不完整的基体上。当鼠伤寒沙门氏菌的钩蛋白基因flaFV被克隆到多拷贝质粒pBR322上并导入这些突变体时，细胞将钩和丝组装到不完整基体上的效率显着增加。这些细胞在半固体平板上形成特征性的点状群，表明携带无外环鞭毛的细胞由于其鞭毛功能差、每个细胞鞭毛数量低或同时存在这两种缺陷而活动能力较弱。 FlaV突变体也产生缺乏外环的不完整基体，但即使在引入携带鼠伤寒沙门氏菌flaFV的质粒后，这些突变体中也没有发生钩和丝的组装。 flaV突变体的失败归因于它们无法将杆尖修改为能够组装钩蛋白的结构。
Among flagellar mutants of Escherichia coli, flaM or flaU mutants form basal bodies lacking the outer P and L rings, whereas flaY mutants predominantly form basal bodies lacking the L ring. In these mutants, hooks and filaments are occasionally assembled onto these incomplete basal bodies. When the hook protein gene, flaFV, of Salmonella typhimurium was cloned on the multicopy plasmid pBR322 and introduced into these mutants, the efficiency with which cells assembled hooks and filaments onto the incomplete basal bodies increased significantly. Such cells formed characteristic dotted swarms on semisolid plates, indicating that cells carrying flagella without the outer rings are weakly motile because of poor function of their flagella, a low flagellar number per cell, or both of these defects. FlaV mutants also produced incomplete basal bodies lacking the outer rings, but assembly of hooks and filaments did not occur in these mutants even after introduction of the plasmid carrying flaFV of S. typhimurium. The failure in the case of flaV mutants was attributed to their inability to modify the rod tip to the structure competent for assembly of hook protein.
DOI：
10.1146/annurev.bb.13.060184.000411
发表时间：
1984
期刊：
Annual review of biophysics and bioengineering
影响因子：
--
作者：
Macnab,RM;Aizawa,S
通讯作者：
Aizawa,S