Real-time and automatic sorting of multi-neuronal activity for sub-millisecond interactions in vivo
Real-time and automatic sorting of multi-neuronal activity for sub-millisecond interactions in vivo
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DOI：
10.1016/j.neuroscience.2005.03.031
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Sakurai, Y
中科院分区：
文献类型：
--
作者：
Takahashi, S;Sakurai, Y
最近的体外电生理学研究表明，相邻的中间神经元通过间隙连接在亚毫秒的时间范围内相互作用，并且各个树突区室在单个神经元内产生局部激发尖峰和反向传播尖峰。然而，大多数使用行为动物的体内电生理学研究仅关注单个神经元和/或大型神经元群体的活动率，而没有考虑神经元之间这种亚毫秒相互作用的潜在作用。这种忽视是由于应用于行为动物的普通体内多神经元记录和尖峰分类技术的限制。尽管独立成分分析（ICA）是克服某些局限性的有效方法，但ICA有一个严重的问题，即单电极（微线）的数量必须多于要记录的单神经元的数量。我们最近开发的方法解决了ICA的这一局限性，但仍然存在一些问题：计算量大，该方法只能用于离线而非实时处理，并且电极神经元漂移问题仍未解决。在本文中，为了解决所有这些问题，我们介绍了一种新颖的系统，该系统由 ICA 结合新开发的多电极十二极自动实时分选组成。该系统有潜力回答一些尚未在体内电生理实验中探索的重要神经生物学问题：紧密相邻的单神经元之间的亚毫秒相互作用如何在自由行为的动物中发挥作用。该系统有望成为连接体外和体内电生理学研究的桥梁。 (c) 2005 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Recent in vitro electrophysiological studies have revealed that neighboring interneurons interact with each other in a sub-millisecond time range via gap junctions and that individual dendritic compartments generate local excitation spikes and back-propagated spikes within a single-neuron. However, most in vivo electrophysiological studies using behaving animals only focus on activity rates of single-neurons and/or large neuronal populations without considering the potential role of such sub-millisecond interactions among neurons. This neglect is due to the limitation of ordinary in vivo multi-neuronal recording and spike sorting techniques applied to behaving animals. Though independent component analysis (ICA) is a powerful method to overcome certain limitations, ICA has a serious problem in that the number of single-electrodes (microwires) must be more than the number of single-neurons to be recorded. Our recently-developed method has solved this limitation of ICA, but a few problems have remained: the computational load is heavy, the method can be used only for off-line, not real-time, processing, and the electrode-neuron drift problem remains unsolved. In this paper, solving all these problems, we introduce a novel system consisting of automatic and real-time spike sorting with ICA in combination with a newly developed multi-electrode, dodecatrode. The system has the potential to answer some important neurobiological questions that have not been explored in in vivo electrophysiological experiments: how sub-millisecond interactions between closely neighboring single-neurons act in freely behaving animals. The system promises to be a bridge connecting electrophysiological studies in vitro and in vivo. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.