Regions in Bacteroides plasmids pBFTM10 and pB8-51 that allow Escherichia coli-Bacteroides shuttle vectors to be mobilized by IncP plasmids and by a conjugative Bacteroides tetracycline resistance element
Regions in Bacteroides plasmids pBFTM10 and pB8-51 that allow Escherichia coli-Bacteroides shuttle vectors to be mobilized by IncP plasmids and by a conjugative Bacteroides tetracycline resistance element
复制标题
拟杆菌属质粒 pBFTM10 和 pB8-51 中允许大肠杆菌-拟杆菌属穿梭载体被 IncP 质粒和接合拟杆菌属四环素抗性元件动员的区域
作者：
N. Shoemaker;C. Getty;E. Guthrie;A. Salyers
用IncP质粒R751或pRK231（RK2的氨苄青霉素敏感性衍生物）在大肠杆菌之间移动含有不可移动的pBR 322衍生物和pBFTM10（pDP 1，pCG 30）或pB8 - 51（pEG 920）的类杆菌-大肠杆菌穿梭载体。coli菌株和E.大肠杆菌转化为拟杆菌受体。IncI alpha R64 drd-ll转移这些载体的效率比IncP质粒低1，000倍。pDP 1、pCG 30和pEG 920可从B中被动员出来。uniformis的供体，大肠杆菌和类杆菌受体通过接合类杆菌Tcr（Tcr ERL）元件连接，该元件最初发现于临床脆弱类杆菌菌株（B. fragilis ERL）。然而，穿梭载体pE5 - 2（其含有克隆在限制性位点中的pB8 - 51，该限制性位点防止其被IncP或IncI α质粒动员）也没有以可检测的频率被Tcr ERL元件从拟杆菌供体中动员。B中Tcr ERL元件对pCG 30、pDP 1和pEG 920的动员频率。uniformis供者为E.大肠杆菌受体与同基因B受体的感染率基本相同。uniformis接收器。从B转移穿梭载体。uniformis供者为E.大肠杆菌在需氧和厌氧条件下发生的频率相同。B的增长。在偶联之前，在四环素（1微克/ml）中的单胞菌供体增加了载体对两种E. coli和Bacteroides受体50~100倍。
Bacteroides-Escherichia coli shuttle vectors containing a nonmobilizable pBR322 derivative and either pBFTM10 (pDP1, pCG30) or pB8-51 (pEG920) were mobilized by IncP plasmid R751 or pRK231 (an ampicillin-sensitive derivative of RK2) between E. coli strains and from E. coli to Bacteroides recipients. IncI alpha R64 drd-ll transferred these vectors 1,000 times less efficiently than did the IncP plasmids. pDP1, pCG30, and pEG920 could be mobilized from B. uniformis donors to both E. coli and Bacteroides recipients by a conjugative Bacteroides Tcr (Tcr ERL) element which was originally found in a clinical Bacteroides fragilis strain (B. fragilis ERL). However, the shuttle vector pE5-2, which contains pB8-51 cloned in a restriction site that prevents its mobilization by IncP or IncI alpha plasmids, also was not mobilized at detectable frequencies from Bacteroides donors by the Tcr ERL element. The mobilization frequencies of pCG30, pDP1, and pEG920 by the Tcr ERL element in B. uniformis donors to E. coli recipients was about the same as those to isogenic B. uniformis recipients. Transfer of the shuttle vectors from B. uniformis donors to E. coli occurred at the same frequencies when the matings were done aerobically or anaerobically. Growth of the B. uniformis donors in tetracycline (1 microgram/ml) prior to conjugation increased the mobilization frequencies of the vectors to both E. coli and Bacteroides recipients 50 to 100 times.
肠道拟杆菌属中三个同源小隐秘质粒的鉴定。
影响因子：
2.6
作者：
Callihan,DR;Young,FE;Clark,VL
通讯作者：
Clark,VL