Detection and resolution of genetic loci affecting circadian period in Brassica oleracea
Detection and resolution of genetic loci affecting circadian period in Brassica oleracea
复制标题
影响甘蓝昼夜节律的基因位点的检测和解析
DOI：
10.1007/s00122-006-0468-y
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发表时间：
2007
影响因子：
5.4
通讯作者：
G. King
中科院分区：
文献类型：
--
作者：
N. Salathia;J. Lynn;A. Millar;G. King
昼夜节律调节植物生长、健康和活力的许多方面。到目前为止，在参考物种拟南芥中已经剖析了昼夜节律调节的组成和详细的机制。为了确定甘蓝近缘子叶内在昼夜节律的遗传基础和自然等位基因变异的范围，我们使用一个准确和高通量的数据捕获系统记录了来自不同作物类型亲本的两个甘蓝不朽分离群体的子叶有节奏的运动。在∼3.5h的两个群体中，两个群体中的极端重组系之间存在超亲分离，每个群体中单个QTL的加性效应在0.17~0.36h之间变化，验证了在一个加倍单倍体群体中检测到的QTL，并通过测定来自亲本的基因组替代系的昼夜节律来确定作图间隔。基于油菜和拟南芥之间共线性的比较基因组分析也可以确定已知的在拟南芥中调节周期的候选同源基因，这可能解释了特定QTL的相加昼夜效应。在两个群体中检测到的不同的QTL位置和超亲分离的程度表明，在天然群体和油菜属作物物种中可能有相当大的空间来调节可用昼夜节律的范围。这可能为优化不同纬度、不同季节或不同气候条件下的生长和发育提供适应性优势。
Circadian rhythms regulate many aspects of plant growth, fitness and vigour. The components and detailed mechanism of circadian regulation to date have been dissected in the reference species Arabidopsis thaliana. To determine the genetic basis and range of natural allelic variation for intrinsic circadian period in the closest crop relatives, we used an accurate and high throughput data capture system to record rhythmic cotyledon movement in two immortal segregating populations of Brassica oleracea, derived from parent lines representing different crop types. Periods varied between 24.4 and 26.1 h between the parent lines, with transgressive segregation between extreme recombinant lines in both populations of ∼3.5 h. The additive effect of individual QTL identified in each population varied from 0.17 to 0.36 h. QTL detected in one doubled haploid population were verified and the mapping intervals further resolved by determining circadian period in genomic substitution lines derived from the parental lines. Comparative genomic analysis based on collinearity between Brassica and Arabidopsis also allowed identification of candidate orthologous genes known to regulate period in Arabidopsis, that may account for the additive circadian effects of specific QTL. The distinct QTL positions detected in the two populations, and the extent of transgressive segregation suggest that there is likely to be considerable scope for modulating the range of available circadian periods in natural populations and crop species of Brassica. This may provide adaptive advantage for optimising growth and development in different latitudes, seasons or climate conditions.
DOI：
10.1073/pnas.96.7.4176
发表时间：
1999-03-30
影响因子：
11.1
作者：
Green, RM;Tobin, EM
通讯作者：
Tobin, EM