Identification of Trichophyton rubrum by nested PCR analysis from paraffin embedded specimen in trichophytia profunda acuta of the glabrous skin.
Identification of Trichophyton rubrum by nested PCR analysis from paraffin embedded specimen in trichophytia profunda acuta of the glabrous skin.
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DOI：
10.3314/jjmm.46.129
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Nishikawa, Takeji
中科院分区：
文献类型：
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作者：
Nagao, Keisuke;Sugita, Takashi;Nishikawa, Takeji
一名67岁的日本男性出现无毛皮肤急性深毛癣菌（TPAGS）。患者左前臂出现硬化红斑斑块和结节。KOH制剂中的鳞屑的直接显微镜检查对真菌成分呈阴性，皮肤真菌培养也呈阴性。虽然在苏木精-伊红染色切片中不能证实真菌感染，但PAS染色活检皮肤病变的深切切片显示真菌成分的外发存在。用从福尔马林固定、石蜡包埋的皮肤切片获得的模板DNA，用针对内部转录间隔区基因1（ITS 1）的毛癣菌特异性引物进行巢式PCR。与T.获得红色，从而鉴定病原体。KOH测试和文化可能往往不成功，也许反映了毛囊占主导地位的真菌生长TPAGS。虽然这些测试是最重要的TPAGS的诊断，巢式PCR石蜡包埋的皮肤切片可能是一种替代方法来确定病原体。
Trichophytia profunda acuta of the glabrous skin (TPAGS) arose in a 67-year-old Japanese man. The patient presented indurated erythematous plaques and nodules on his left forearm. Direct microscopic examination of the scale in KOH preparation was negative for fungal elements, and culture for dermatophytes was also negative. Although fungal infection could not be proven in hematoxillin-eosin stained sections, deep-cut sections of the biopsied skin lesion with PAS stain revealed the ectothrix presence of fungal elements. Nested PCR was done with Trichophyton specific primers directed to internal transcribed spacer gene 1 (ITS1), using template DNA obtained from formalin fixed, paraffin embedded skin sections. A single band corresponding to T. rubrum was obtained, and the etiological agent was thus identified. KOH tests and cultures may often turn out unsuccessful, perhaps reflecting the hair follicle dominant fungus growth in TPAGS. Although these tests are most important for diagnosis of TPAGS, nested PCR using paraffin embedded skin sections may be an alternative method to identify the etiological agent.