Direct tracking of pollen with quantum dots reveals surprising uniformity in dispersal distance across 11 populations of an annual plant
Direct tracking of pollen with quantum dots reveals surprising uniformity in dispersal distance across 11 populations of an annual plant
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用量子点直接追踪花粉揭示了一年生植物 11 个种群的传播距离惊人的均匀性
DOI：
10.1002/ajb2.16201
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发表时间：
2023
影响因子：
3
通讯作者：
Moeller, David A.
中科院分区：
文献类型：
--
作者：
Kern, Brooke R.;Carley, Lauren N.;Moeller, David A.
花粉运动是种子植物传播的重要组成部分。虽然花粉传播研究得很好，但方法上的限制使得直接跟踪景观中多个种群的花粉流动具有挑战性。利用量子点标记花粉的新技术，研究了Clarkia xantianasubsp.xantiana 11个居群花粉散布的空间尺度及其与种内密度的关系，一种蜜蜂授粉的一年生植物。方法我们在两年的时间里使用实验阵列来跟踪9个种群内5-35米距离的花粉运动，以及10- 15米距离的花粉运动。70米内的两个额外的人口。我们测试的距离衰减的花粉传播，是否同种密度调制的传播距离，以及是否分散内核在整个环境复杂的landscape.ResultsLabeled花粉接收人口之间的变化没有下降，距离超过35米内的8个9个人口或超过70米内的两个人口。花粉接收量随种密度的增加而增加。总体而言，分散内核是一致的population.ConclusionsThe令人惊讶的均匀性，在不同的人群内的分散距离很可能受到低降水量和植物密度在我们的研究年份。这表明非生物环境中的时空变化对种群内和种群间的基因流动程度有很大影响。
PremisePollen movement is a crucial component of dispersal in seed plants. Although pollen dispersal is well studied, methodological constraints have made it challenging to directly track pollen flow within multiple populations across landscapes. We labeled pollen with quantum dots, a new technique that overcomes past limitations, to evaluate the spatial scale of pollen dispersal and its relationship with conspecific density within 11 populations ofClarkia xantianasubsp.xantiana, a bee‐pollinated annual plant.MethodsWe used experimental arrays in two years to track pollen movement across distances of 5–35 m within nine populations and across distances of 10–70 m within two additional populations. We tested for distance decay of pollen dispersal, whether conspecific density modulated dispersal distance, and whether dispersal kernels varied among populations across an environmentally complex landscape.ResultsLabeled pollen receipt did not decline with distance over 35 m within eight of nine populations or over 70 m within either of two populations. Pollen receipt increased with conspecific density. Overall, dispersal kernels were consistent across populations.ConclusionsThe surprising uniformity in dispersal distance within different populations was likely influenced by low precipitation and plant density in our study years. This suggests that spatiotemporal variation in the abiotic environment substantially influences the extent of gene flow within and among populations.